响应面法优化超临界CO_2萃取刺葡萄酒渣中白藜芦醇工艺

Optimization of supercritical CO_2 extraction of resveratrol from V.didii.Foex fruit wine residues by response surface methodology

为提高刺葡萄的综合利用价值,以南方"紫秋"刺葡萄酒渣为原料,采用超临界CO2萃取白藜芦醇。在单因素实验基础上,利用响应面法研究了萃取温度、萃取压力、萃取时间及夹带剂用量对白藜芦醇含量的影响,并优化出超临界CO2萃取刺葡萄酒渣中白藜芦醇的最佳工艺条件为萃取温度32℃,萃取压力365bar,萃取时间1.40h,夹带剂用量3.38m L/g,在此条件下白藜芦醇的含量达387.34μg/g,且以反式白藜芦醇苷的形式存在。

In order to improve comprehensive utilization of V.didii.Foex,the resveratrol from V.didii.Foex fruit wine residues was extracted by supercritical fluid extraction. Based on single factor experiments,response surface methodology was adopted to investigate the effects of extraction temperature,extraction pressure,extraction time and entrainment agent dosage on resveratrol yield. The optimal extraction conditions were as follows：extraction temperature 32℃,extraction pressure 365 bar,extraction time 1.40 h and entrainment agent dosage3.38 m L/g. Under these conditions,the yield of resveratrol was 387.34μg/g,and mainly in the form of trans-piceid.