牛蒡根水提物对高血压大鼠血管内皮损伤的保护作用

Protective effects of aqueous extract of arctium lappa root on vascular endothelial cell injury of hypertensive rats

目的探讨牛蒡根水提物对高血压大鼠血管内皮损伤的保护作用及机制。方法用N-硝基-L-精氨酸(L-NNA)复制高血压大鼠模型,随机分为正常对照组、模型对照组、阳性对照组(卡托普利15 mg/kg)、牛蒡根水提物低剂量组(0.5 g/kg)、牛蒡根水提物中剂量组(1.0 g/kg)及牛蒡根水提物高剂量组(2.0 g/kg),连续灌胃6周,测定大鼠用药后1、4、7、10、13、16、19、22、29、36、42 d的收缩压;6周后,检测大鼠血清C-反应蛋白(CRP)、白介素-6(IL-6)水平,取大鼠胸主动脉,测细胞间黏附分子-1(ICAM-1)水平。结果 (1)牛蒡根水提物组的大鼠尾动脉收缩压低于模型对照组(P<0.05)。(2)牛蒡根水提物可显著改善血管的内皮损伤程度,抑制内膜内皮细胞脱落及血细胞黏附,并抑制中膜平滑肌细胞、胶原纤维增殖等。(3)牛蒡根水提物组的血清IL-6、CRP炎症因子及血管内皮ICAM-1表达水平低于模型对照组(P<0.05)。结论牛蒡根水提物对高血压大鼠血管内皮损伤具有明显改善作用,其机制可能与其抑制炎症因子IL-6、CRP及ICAM-1的表达,改善高血压大鼠血管内皮慢性炎症反应有关。

Objective To study the protective effects and mechanism of aqueous extract of arctium lappa root on vas？cular endothelial cell injury in hypertensive rats. Methods The hypertensive rat model was induced by N-nitro-L-argi？nine. Rats were randomly divided into normal control group, model control group, positive control group （aptopril 15 mg/kg）, low concentration of aqueous extract of arctium lappa root （0.5 g/kg）, medium concentration of （1 g/kg） and high concentra？tion of （2 g/kg） groups. After six weeks of continuous intragastric administration, the systolic blood pressure levels at tail ar？tery were measured at 1, 4, 7, 10, 13, 16, 19, 22, 29, 36 and 42 d after treatment. And other indicators related to inflammato？ry factors were detected including C-reactive protein （CRP） and interleukin （IL）-6. The intercellular adhesion molecule-1 （ICAM-1） level was detected by taking samples of thoracic aorta. Results （1） The systolic blood pressure level at tail ar？tery was significantly lower in aqueous extract of arctium lappa root group than that of model control group （ P〈0.05）. （2） The aqueous extract of arctium lappa root can significantly improve the vascular endothelial cell injury, suppress vascular endothelial cell loss and blood cell adhesion, and cell proliferation with collagen fibers in muscle membrane. （ 3） The serum levels of IL-6, CRP and vascular endothelial ICAM-1 were significantly reduced in aqueous extract of arctium lappa root group than that of model control group （P〈0.05）. Conclusion Aqueous extract of arctium lappa root can significantly improve vascular endothelial cell injury in hypertensive rats. The mechanism may be related to the inhibition of inflammatory cytokines like IL-6, CRP and the expression of ICAM-1, and the improvement of chronic inflammatory response in vascular en？dothelium of hypertensive rats.