Growth effects of botulinum toxin type A injected unilaterally into the masseter muscle of developing rats

咬肌单侧注射A型肉毒毒素对大鼠下颌骨生长发育的影响(英文)

Objective： To evaluate the effects of botulinum toxin type A （BTX-A） on mandible skeletal development by inducing muscle hypofunction. Methods： Four-week-old Sprague-Dawley rats （n=60） were divided into three groups： Group 1 animals served as controls and were injected with saline; Group 2 animals were injected unilaterally with BTX-A （the contralateral side was injected with saline）; and Group 3 animals were injected bilaterally with BTX-A. In Group 2, the saline-injected side was designated the control side （Group 2-1）, whereas the BTX-A-injected side was designated the experimental side （Group 2-2）. After four weeks, the animals were sacrificed, dry skulls were prepared, and mandibles were measured. Results： In the unilateral group, the experimental side （Group 2-2） had reduced di- mensions for all mandible measurements compared with the control side （Group 2-1）, suggesting a local effect of BTX-A on mandible growth, likely due to muscle reduction. Conclusions： Localized BTX-A injection induced a change in craniofacial growth, and the skeletal effect was unilateral despite both sides of the mandible functioning as one unit.

目的:通过诱导肌肉功能衰退,评估A型肉毒毒素(BTX-A)对下颌骨生长发育的影响。创新点:通过对同一个体的对照侧(组2-1)和实验侧(组2-2)下颌骨尺寸的测量分析,证明BTX-A对下颌骨生长具有局部效应。方法:四周龄的Sprague-Dawley大鼠(60只)随机分为三组:第1组动物注射生理盐水作为对照组;第2组动物单侧注射BTX-A(对侧注射生理盐水);第3组动物双侧注射BTX-A。在第2组中,注射生理盐水的一侧作为对照侧(组2-1),而BTX-A注射的一侧作为实验侧(组2-2)。四周后,处死动物,制备干头骨,测量下颌骨。结论:尽管下颌骨的两侧作为一个功能单元,但是局部注射BTX-A诱导产生的颅面生长改变以及对骨骼的影响却是单侧的。