摘要
目的研究TLR7在胰腺癌Bx PC-3细胞中表达,并探讨TLR7激动剂激活TLR7后细胞因子白介素15(IL-15)的表达。方法通过Western blot和Real-time PCR分析TLR7在细胞内的表达水平;Bx PC-3细胞经过不同时间点Gardiquimod(3μg/ml)的处理后,Real-time PCR分析TLR7激活后IL-15 mRNA水平表达变化;Western blot分析Gardiquimod刺激细胞后,磷脂酰肌醇-3-激酶-蛋白质丝氨酸苏氨酸激酶(PI3K-AKT)信号通路的变化。结果 Western blot和Real-time PCR结果显示:与外周血单核细胞(PBMC)相比,TLR7在Bx PC-3中弱表达;Real-time PCR分析显示:Gardiquimod处理细胞后能刺激细胞中IL-15的表达;TLR7激动剂能够激活PI3K-AKT信号通路。结论 Gardiquimod激活TLR7后能够上调IL-15的表达,并且其激活与PI3KAKT信号途径相关。
Objective To investigate the expression of Toll-like receptor 7 （ TLR7 ） in pancreatic cancer BxPC-3 cells, and to explore the effect of TLR7 activation on the expression of interleukin-15（IL-15）. Methods BxPC-3 cells were used to analyze the expression of TLR7 by Western blot and Real-time PCR. The cells were treated with Gardiquimod （3 μg/ml） at different times, the expression of IL-15 at mRNA level by Real-time PCR. Western blot was performed to analyze the phosphorylation level changes of phosphatidyl inositol kinase serine/threonine kinase （PI3K-AKT） protein in BxPC-3 cells stimulated by TLR7 ligand Gardiquimod. Results Western blot and Real-time PCR results showed that compared with peripheral blood mononuclear cells （ PBMC ） , TLR7 presented weak expression in BxPC-3 cells. Gardiquimod could increase the expression of IL-15 in BxPC-3 cells. TLR7 agonist could activate the PI3K-AKT signaling pathway. Conclusion Gardiquimod can up-regulate the expression of IL-15 and this effect can be associated with PI3K-AKT signaling pathway.
出处
《安徽医科大学学报》
CAS
北大核心
2015年第1期1-4,共4页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金(编号:81271748)
