p22-phox在P19细胞向心肌细胞分化前后的变化

The changes of the expression of p22-phox before and after P19 cells differentiating into cardiac myocytes

目的探讨P19细胞向心肌细胞分化前后p22-phox水平的变化。方法贴壁培养P19细胞,取合适细胞株,应用0.9%二甲基亚砜(DMSO)诱导培养,收集诱导不同时间的细胞,通过Western blot检测其肌钙蛋白I(c Tn I)水平,以确定分化,并行Western blot检测P19细胞向心肌细胞分化前后细胞中p22-phox蛋白水平。结果1 P19细胞经0.9%DMSO诱导分化后,于第8天开始检测到c Tn I表达阳性,后c Tn I表达增加,差异有统计学意义(P<0.05)。2P19细胞向心肌细胞分化后细胞内p22-phox水平较分化前高,差异有统计学意义(P<0.05)。结论 p22-phox在P19细胞向心肌细胞分化后表达增加,氧化应激水平增高。

Objective To investigate the change of the expression of p22-phox before and after P19 cells differenti-ating to cardiac myocytes. Methods Appropriate P19 cells were chosen which were cultured in vitro with 0. 9%dimethyl sulfoxide（ DMSO） , then the induced cells were collected at different times. Western blot of cardiac tropo-nin I （ cTnI） was used to identify cell differentiation, and detect the level of p22-phox before and after the differen-tiation. Results ① On the 8th day cTnI was detected positive in P19 cells which were cultured with 0. 9% DM-SO, then the expression of cTnI was increased, with statistical significance（P〈0. 05）.② The expression of p22-phox in differentiated P19 cells was higher than that in undifferentiated P19 cells, with statistical significance（ P〈0. 05） . Conclusion The expression of p22-phox increases during the differentiation of P19 cells to cardiac myo-cytes, and the level of oxidative stress increases.