不同浓度乙醇对TNBS诱导小鼠克罗恩病模型的影响

The effect of different concentrations of ethanol on mice Crohn's disease model induced by TNBS

建立2,4,6-三硝基苯磺酸(TNBS)小鼠克罗恩病(CD)模型,通过观察小鼠每日表现、存活率、疾病活动指数(DAI)、结肠肉眼观和组织学变化及肠道纤维化情况,筛选出造模所需最佳乙醇浓度。各模型组小鼠均不同程度出现CD的一般表现。随着乙醇浓度增加,小鼠DAI评分、肠道病理及纤维化评分逐渐增高,以TNBS/50%乙醇组最为显著,最类似人类CD病理改变。50%为造模所需最佳乙醇浓度,其配比2.0 mg TNBS诱导的模型成模率高且小鼠生存率适中,较适宜用作CD发病机制研究和新靶点的探索。

Crohn’s disease（CD）mice model was induced by 2, 4, 6-trinitrobenzene sulfonic acid（TNBS）. The daily performance, survival rate, disease activity index （ DAI） , colon morphologic and histologic changes and in-testinal fibrosis degree of mice were observed in order to investigate the optimal ethanol concentration. Mice in mod-el groups all performed general symptoms of CD in different degrees. The grades of mice DAI, histology and intesti-nal fibrosis increased gradually with the increase of ethanol concentrations, and the TNBS/50% ethanol group was most significant which was most similar to human CD pathological changes. An ethanol concentration of 50% for mice was chosen for an optimal experimental concentration since the group had the highest success rate of modeling when matched with 2. 0 mg TNBS and showed appropriate survival rate, which was more suitable for the investiga-tion of pathogenesis and new therapeutic targets of CD.