摘要
目的探讨左旋乳酸-聚己内酯共聚物和纤维蛋白原共混静电纺盆底修复材料应用于盆底脏器脱垂治疗中的体内降解和生物再生特性的研究。方法在30只SD大鼠左右两侧腹壁建立腹壁缺损区,利用静电纺盆底修复材料对缺损进行修补,一组为补片平铺修补,另一组补片褶皱后修补。术后1、4、8、12、16周各处死6只大鼠,取出修补区域及其周围组织,通过测定聚合物分子量、抗拉强度及电镜观察分析补片在体内的降解、组织的病理变化及再生情况。结果降解16周后,两组机械强度差异无统计学意义,但具有可比性;褶皱组相对分子量大于平铺组(P<0.05);电镜扫描结果显示两组有明显差异,平铺组补片降解速度快,但无法维持一定的力学强度,褶皱组降解速度慢,但其可保持一定的力学强度;HE染色、电镜扫描结果都证实两组具有差异性。结论通过两种实验方法对比后发现,平铺组降解虽快,但却不能维持一定的力学强度,因此褶皱植入法优于平铺植入法。
Objective To study the characteristics of intracorporeal degradation and regeneration of electro-spun pelvic floor repair materials composed of LLA-CL and fibrinogen. Methods The abdominal wall defects were created in the left and right sides of the abdominal spaces of 30 SD rats. Then the defects were repaired by electrostatic spinning pelvic floor repair materials. The smooth group was paved followed by repair, and the other group was ruffled before repair. Six rats were sacrificed in each zone of 1, 4, 8, 12, 16 weeks after the operation. The next step was to take out the repaired parts and the surrounding tissues and analyzed the degradation of mesh, the pathologic changes and regeneration of the organization by calculating the molecular weight and the mechanical strength and electron microscopy observation. Results After sixteen weeks degradation, there was no statistically significant difference between the mechanical strength of the two groups, but the results were comparable. The relative molecular weight of the ruffle group surpassed the smooth group （P 〈 0.05）. At the same time, HE staining and electron microscope scanning results have proved that differences existed between the two groups. The speed of patch degradation is faster in the smooth group, but it is unable to maintain a certain mechanical strength. The slow degradation of the ruffle group can keep a certain mechanical strength. Conclusion By comparison with two kinds of experimental methods, we find that the ruffled implantation is better than that of the smooth implants.
出处
《中国医药生物技术》
2015年第1期45-52,共8页
Chinese Medicinal Biotechnology
