人乳头瘤病毒基因型与宫颈病变的相关性分析

Correlation analysis of human papilloma virus genotypes and cervical lesions

目的探讨不同基因型人乳头瘤病毒(human papilloma virus,HPV)感染与宫颈病变的相关性。方法采用导流杂交基因芯片技术(Hybrimax)对924例妇女进行21种HPV基因型检测,同期进行宫颈活检病理检查。按病理学诊断结果分成6组:宫颈鳞癌组287例;宫颈上皮内瘤变(CIN)Ⅲ组78例;CINⅡ组61例;CINⅠ组153例;宫颈湿疣组40例;正常或炎症组305例。结果⑴503例检测到HPV感染,阳性率为54.4%。所有标本共检测到19种HPV基因型,未检测到HPV43、44型。不同病变组HPV阳性率差异有统计学意义(P<0.05),宫颈鳞癌组与宫颈湿疣组HPV阳性率显著高于CINⅢ组(P<0.05),CINⅢ组HPV阳性率显著高于CINⅡ组与CINⅠ组(P<0.05),CINⅡ组与CINⅠ组HPV阳性率显著高于正常或炎症组(P<0.05)。⑵不同病变组HPV多重感染率差异有统计学意义(P<0.05),宫颈鳞癌组、CINⅢ组、CINⅡ组、CINⅠ组HPV多重感染率显著高于湿疣组与正常或炎症组(P<0.05)。结论不同类型宫颈病变与HPV感染率、HPV感染型别有着一定的关系,HPV多重感染可能是宫颈鳞癌以及宫颈上皮内瘤变的危险因素。

Objective To investigate the correlation between the infection of different genotypes of human papilloma virus（HPV） and cervical lesions. Methods Twenty-one kinds of HPV genotypes were detected in 924 cases of women by flow-through hybridization and gene chip technology, and the cervical biopsy was performed at the same time. According to the results of pathological diagnosis, these women were divided into 6 groups, including cervical squamous cell carcinoma group（287 cases）, cervical intraepithelial neoplasia（CIN）Ⅲ group（78 cases）, CINⅡ group（61 cases）, CINⅠ group（153 cases）, cervical condyloma group（40cases）, normal or inflammation group（305 cases）. Results A total of 503 cases were HPV-positive, and the positive rate was54.4%. 19 kinds of HPV genotypes were detected, except HPV43 and HPV44. There were statistical differences in the infection rates of HPV among the 6 groups（P〈0.05）, and the infection rate of HPV in cervical squamous cell carcinoma group or cervical condyloma group was significantly higher than that in CINⅢ group（P〈0.05）, and the infection rate in CINⅢ group was significantly higher than that in CINⅡ group or CINⅠ group（P〈0.05）, the infection rate of HPV in CINⅡ group or CINⅠ group was significantly higher than that in normal or inflammation group（P〈0.05）. ⑵ There were statistical differences in the multiple infection rates of HPV among the 6 groups（P〈0.05）, the multiple infection rates of HPV in cervical squamous cell carcinoma group, CINⅢ group, CINⅡ group or CINⅠ group were significantly higher than that in cervical condyloma group or normal or inflammation group（P 0.05）.Conclusion There is a certain relationship betweeen different kinds of cervical lesions and infection rates of HPV, as well as HPV genotype. HPV multiple infection may be a risk factor for cervical squamous cell carcinoma and cervical intraepithelial neoplasia.