摘要
目的：探讨麻黄细辛附子汤对变应性鼻炎大鼠血浆白细胞介素-6（interlcukin-6, IL-6）、肿瘤坏死因子-α（tumor necrosis factor-α, TNF-α）的影响。方法32只Wistar大鼠按按体质量采用随机区组法分成正常对照组、模型组、氯苯那敏组和麻黄细辛附子汤组，每组8只。采用卵清蛋白致敏制作变应性鼻炎模型。麻黄细辛附子汤组麻黄细辛附子汤灌胃（0.4 g），氯苯那敏组灌胃0.01%氯苯那敏溶液，模型组和正常对照组等体积生理盐水灌胃，2次/d，共21 d。采用免疫吸附法检测血浆IL-6、TNF-α水平。结果麻黄细辛附子汤组喷嚏[（2.4±0.9）次/30 min比（18.5±3.6）次/30 min]、鼻抓痒[（4.2±1.5）次/30 min比（31.2±3.7）次/30 min]频率显著低于模型组（P 均＜0.05）。麻黄细辛附子汤组血浆 IL-6[（153.00±13.87）pg/ml 比（230.38±33.92）pg/ml]、TNF-α[（21.25±4.62）pg/ml 比（56.38±7.93）pg/ml]水平显著低于模型组（P均＜0.05）。结论麻黄细辛附子汤可降低血浆IL-6与TNF-α水平，减轻大鼠变应性鼻炎症状。
Objective To investigate the effects of Mahuang-Xixin-Fuzi decoction on the plasma levels of interlcukin-6 （IL-6） and tumor necrosis factor-α （TNF-α） in allergic rhinitis in rats. Methods Thirty-two Wistar rats were randomly divided into a control group, a model group, a chlorpheniramine group, and a Mahuang-Xixin-Fuzi decoction group, with 8 rats in each group. Allergic rhinitis was induced by ovalbumin sensitization. Rats in the chlorpheniramine and Mahuang-Xixin-Fuzi decoction groups received intragastric administration of 0.01%chlorpheniramine and Mahuang-Xixin-Fuzi decoction 0.4 g （twice daily for 21 days）, respectively. The plasma levels of IL-6 and TNF-αwere measured with enzyme-linked immunosorbent assay. Results The frequencies of sneezing （2.4 ± 0.9/30 min vs.18.5 ± 3.6/30 min） and nasal rubbing （4.2 ± 1.5/30 min vs. 31.2 ± 3.7/30 min） in the Mahuang-Xixin-Fuzi decoction group were significantly lower than those in the model group （all P〈0.05）. The plasma levels of IL-6 （153.00 ± 13.87 pg/ml vs. 230.38 ± 33.92 pg/ml） and TNF-α（21.25 ± 4.62 pg/ml vs. 56.38 ± 7.93 pg/ml） in the Mahuang-Xixin-Fuzi decoction group were significantly lower than those in the model group （all P〈0.05）. Conclusions Mahuang-Xixin-Fuzi decoction can reduce the plasma levels of IL-6 and TNF-α, and alleviate symptoms in allergic rhinitis in rats.
出处
《国际中医中药杂志》
2015年第2期153-156,共4页
International Journal of Traditional Chinese Medicine
