摘要
Caragana microphylla is the most dominant and constructive shrub species in the Horqin Sandy Land of northeastern China. We evaluated the level of genetic variation within and among C. microphylla populations sampled from three different temperature gradients in the Horqin Sandy Land by using inter-simple sequence repeat polymorphism （ISSR） molecular markers. The results show that eight ISSR primers generated 127 bands, of which 123 （96.85%） were polymorphic. At the species level, genetic diversity was relatively high （P = 96.85%, h = 0.3143, I = 0.4790）. The highest genetic diversity was observed in the Subp6 population from low temperature regions, whereas the lowest diversity was found in the Subp2 population from high temperature regions. Six populations of C. microphylla clustered into two clades. These results have important implications for restoring and managing the degraded ecosystem in arid and semi-arid areas.
Caragana microphylla is the most dominant and constructive shrub species in the Horqin Sandy Land of northeastern China. We evaluated the level of genetic variation within and among C. microphylla populations sampled from three different temperature gradients in the Horqin Sandy Land by using inter-simple sequence repeat polymorphism （ISSR） molecular markers. The results show that eight ISSR primers generated 127 bands, of which 123 （96.85%） were polymorphic. At the species level, genetic diversity was relatively high （P = 96.85%, h = 0.3143, I = 0.4790）. The highest genetic diversity was observed in the Subp6 population from low temperature regions, whereas the lowest diversity was found in the Subp2 population from high temperature regions. Six populations of C. microphylla clustered into two clades. These results have important implications for restoring and managing the degraded ecosystem in arid and semi-arid areas.
基金
financially supported by One Hundred Person Project of the Chinese Academy of Sciences and research projects--41201561,2011BAC07B02,Y351151001 and 145RJYA269
