炔雌醚对布氏田鼠繁殖的抑制效果

Suppression effects of quinestrol on the reproduction of Brandt's voles (Lasiopodomys brandtii)

明确炔雌醚对鼠类繁殖抑制最适剂量及持续有效作用时间,对其后续相关理论研究和实践应用具有基础性作用。本研究以0.5 mg/kg、1.0 mg/kg、2.0 mg/kg、3.0 mg/kg、6.0 mg/kg浓度炔雌醚分别连续灌胃雄性和雌性布氏田鼠7 d后,与正常异性配对观察90 d。按繁殖启动期、繁殖率和繁殖力等最直观的指标综合评价了5个浓度炔雌醚对雌雄布氏田鼠的不育效果。室内条件下,炔雌醚能够延迟雌雄布氏田鼠的生殖启动,但可能由于样本量小,未达统计显著性。90 d内,一定浓度的炔雌醚能够降低雌雄个体的繁殖率和平均每窝产仔数,同样未达统计显著性。一些浓度的药物能够显著降低布氏田鼠的繁殖力,其抑制效果无剂量相关性。推断炔雌醚对雄性布氏田鼠的最适不育作用浓度为2.0 mg/kg左右,对雌性的最适不育作用浓度为1.0 mg/kg左右。实际综合应用剂量2.0 mg/kg左右,能有效降低布氏田鼠产仔总数,其持续时间可达90 d。

It is critical to confirm the optimum dose and the active duration of the sterilization by quinestrol on rodents for some studies and practices. We fed female and male Brandt＇s voles 5 doses of 0. 5 mg / kg,1. 0 mg / kg,2. 0 mg / kg,3. 0 mg / kg,or 6. 0 mg / kg quiestrol for 7 consecutive days. We then assessed their anti-fertility effects on the voles by analyzing reproduction indices including birth date,reproductive rate and reproductive capacity. Results showed that quinestrol delayed the breeding of Brandt＇s voles in the lab,but the effect was not significant due to the small sample size. In 90 days,some doses of quinestrol decreased the reproductive rate and average litter size of female and male,however,the inhibitory effect still was not significant,again possibly due to small sample size; some doses of quinestrol decreased markedly the reproductive capacity of Brandt＇s voles. The suppression effects were not significantly correlated with the doses of quinestrol. The optimum doses of quinestrol causing sterility were below 2. 0 mg / kg for male and 1. 0 mg / kg for female. The dose applyed for Brandt＇s vole should be below 2. 0 mg / kg,which can reduce total litter size of Brandt＇s vole obviously within 90 days.