海洋石油降解菌的筛选、鉴定及降解活性

Isolation and Identification of Petroleum Degrading Marine Bacteria and Its Activity

从渤海入海口的滩涂采集泥沙,采用石油为唯一碳源的筛选培养基,梯度稀释法与划线平板法作为筛选方法,分离、筛选出1株可降解石油的菌株SI-JHS。经过16S r DNA测序及Biolog 95种碳源分析,鉴定该菌株为假交替单胞菌(Pseudoalteromon sp.)。通过改变p H值、温度、盐度和石油含量等实验对SI-JHS进行石油降解试验。研究表明,菌株SI-JHS的最适宜降解条件为:盐度3.5%,p H值7.0,温度35℃,在该条件下,SI-JHS对石油降解率可达75.71%。

A novel petroleum-degrading microbial strain, designated SI-JHS, was isolated from tidal fiat sediment in estuary of Bohai, by using serial dilution method and plate streak method in the medium containing petroleum as a sole carbon source. On the basis of 16S rRNA gene sequence analysis and Biolog analysis of 95 carbon sources, the strain was primarily identified as Pseudoalteromon. sp. Effects of pH values, temperature, salinity, concentrations of petroleum on degradation of activities SI-JHS were investigated. The results showed that under the optimal growth conditions, with salinity of 3.5% , pH of 7.0, temperature of 35 ℃ , the petroleum could be degraded in the high efficiency of 75.71%.