磷脂酶D催化高温降解后大豆磷脂合成磷脂酰丝氨酸工艺研究

Synthesis of phosphatidylserine catalyzed by phospholipases D from crude soybean lecithin degraded by high-temperature

研究以粗大豆卵磷脂为原料,经过高温降解后采用含磷脂酶D发酵液催化合成磷脂酰丝氨酸的工艺。为了确定高温降解粗大豆卵磷脂的最佳工艺,设计了相应的正交试验。结果表明,通过正交试验优化,以1倍体积水饱和的乙酸丁酯为溶剂,127℃降解40 h能够有效改变粗大豆卵磷脂中其他磷脂的结构和化学性质,有利于磷脂酰胆碱的进一步纯化。通过甲醇提纯后进一步通过磷脂酶D发酵液催化合成磷脂酰丝氨酸,最后采用无水乙醇作为产品的提纯溶剂进行分离纯化,最终得到磷脂酰丝氨酸含量为48.45%的产品。

The crude soybean lecithin were used as the raw material, and were treated with high temperature degradation then the reaction of synthesis phosphatidylserine（PS） was catalyzed by phospholipase D（PLD）. The best method of the thermal degradation for crude soybean lecithin was determined by orthogonal design. Results showed that the best method was determined as follow： the same volume of water saturated n-butyl acetate were used as solvent, degradation 40 hours at 127 ℃. The method of high temperature degradation could change the structures and chemical property of other components, that is useful for further purification of phosphatidylcholine（PC）. The thermal degradation product was further extracted by methanol and catalyzed by phospholipase D（PLD） to obtain phosphatidylserine（PS）. The crude product was purified by ethanol finally, and the content of phosphatidylserine（PS） in the final product was 48.45%.