响应面法优化熟地黄总黄酮的纤维素酶法提取工艺

Optimization of cellulase hydrolysis extraction of total flavonoids from Radix Rehmanniae Preparata by response surface methodology

为优化纤维素酶法辅助提取熟地黄中总黄酮的提取方法,在单因素试验基础上,选取酶浓度、酶解温度及酶解时间为自变量,熟地黄总黄酮提取率为响应值,采用响应曲面统计法中Box-Behnken试验设计方法,研究各自变量及其交互作用对总黄酮提取率的影响,采用DesignExpert软件,建立了总黄酮提取率与提取过程中各因素的二次多项式模型,并通过响应面优化法分别确定在p H为4.5的条件下熟地黄总黄酮提取最佳工艺为:酶浓度80 U/m L、酶解温度38℃,酶解时间48 min,在此修正条件下,提取总黄酮的预测值为2.86%,经过试验验证,熟地黄总黄酮的提取率为2.89%,与预测值的相对误差为1.05%,验证了数学模型的有效性。该优化工艺合理可行,可为熟地黄总黄酮提取提供一定的理论指导和参考依据。

In order to optimize the extraction method of total flavonoids from Radix Rehmannia glutinosa by cellulose-assisted extraction, based on single factor experiments, enzyme concentration, hydrolysis temperature, hydrolysis time were selected as influencing factors and the yield of total flavonoids as response during extraction. The experiment method was designed according to Box-Behnken central composite experiment and each variable and their interactions effects on the yield of total flavonoids were studied. The quadratic multinomial model on the yield of total flavonoids and each factor was established by using the Design-Expert software. The optimum reactive condition of extracting process was determined by means of response surface method when the p H kept in 4.5. The results showed that the optimum conditions for total flavonids were enzyme concentration 80 U/m L, hydrolysis temperature 38 ℃ and hydrolysis time 48 min, respectively. Under this condition, the extraction yield of total flavonids was 2.89% and the relative error was 1.05% which compared to the predictive value being of 2.86%. Meanwhile the results indicated that the feasible model fitted well with the experimental data. From this study, it could be concluded that the extraction system was reliable and practical, the certain reference and theoretical guidance for further research can be provided.