大黄素-BSA包被膜片人工抗原制备及其免疫原性与特异性检测

Preparation and immunogenicity and specificity characterization of Emodin-BSA-PVDF membrane artificial antigen

目的:通过大黄素-BSA包被膜片的免疫原性及特异性研究,探讨半抗原-载体包被膜片人工抗原制备法的可行性。方法:先将BSA包被于PVDF膜片,再与大黄素-偶联剂衍生物偶联,制备大黄素-BSA包被膜片,经膜片皮下包埋法免疫大鼠,用大黄素或大黄酚、大黄素甲醚包被CA膜片免疫分析法检测免疫原性和特异性。结果:大黄素-BSA包被膜片的免疫原性高于或等于液相抗原;其抗血清对三种蒽醌类化合物反应的特异性基本一致(P>0.05)。结论:大黄素-BSA包被膜片抗原,具有良好免疫原性和特异性,提示用半抗原-载体包被膜片法制备人工抗原可行。

Objective： To study feasibility of preparing artificial antigen by membrane coated with hapten-carrier. To compare the Emodin-BSA membrane antigen immunogenicity and specificity against the liquid antigen. Methods： Emodin-BSA-PVDF membrane was prepared by the method that BSA was coated on PVDF membrane and the BSA was coupled with Emodin-couplint agent derivative. Rats were immunized by subcutaneous implantation. The immunogenicity and antibody specificity were characterized using EmodinCA or Chrysophanol-CA or Physcion-CA membrane immunoassay. Results： The immunogenicity of Emodin-BSA coated membrane antigen was higher than Emodin-BSA liquid antigen; the specificity for three anthraquinones was almost the same（ P〉 0. 05）. Conclusion：Emodin antiserum generated using Emodin-BSA coated membrane antigen has a high immunogenicity and specificity to Emodin. The results show it is feasible that membrane coated with hapten-carrier is used as artificial antigen.