小细胞和非小细胞肺癌晚期患者CD3^+CD4^及CD3^+CD8^+T淋巴细胞亚群的差异

Difference of CD3^+ CD4^+ and CD3^+ CD8^+ T cells between patients with advanced small cell lung cancer and non-small cell lung cancer

目的:探索小细胞和非小细胞肺癌晚期患者CD3+CD4+及CD3+CD8+T淋巴细胞亚群是否存在差异,并为治疗提供参考。方法:选取肺癌晚期患者共65例,其中包括小细胞肺癌14例,非小细胞肺癌51例以及20例健康对照。用流式细胞仪检测研究对象外周血淋巴细胞表面CD3+CD4+及CD3+CD8+的表达情况。结果:CD3+CD4+T细胞所占比例无论是小细胞还是非小细胞肺癌晚期的患者都较健康对照显著降低;CD3+CD8+T细胞所占比例在肺癌晚期的患者较健康对照并无显著变化;CD4+/CD8+比值在小细胞肺癌晚期患者较健康对照显著下降。结论:无论是小细胞还是非小细胞肺癌晚期的患者CD3+CD4+T细胞的水平较健康人都显著降低,说明肺癌晚期患者细胞免疫功能严重受损。

Objective： To investigate the difference of CD3＋CD4＋and CD3＋CD8＋T cells between patients with advanced small cell lung cancer and with non-small cell lung cancer,and provide available reference for treatment. Methods： Peripheral blood was taken from 65 patients with advanced lung cancer which was included 14 cases of small cell lung cancer and 51 cases of non-small cell lung cancer,20 cases normal controls. The expression of CD3^＋CD4^＋and CD3＋CD8＋on lymphocytes was analyzed with flow cytometry. Results： We found that the percentage of CD3^＋CD4^＋T cells in small cell and non-small cell lung cancer patients were both much less than that of normal controls. There was no significant variation in the percentage of CD3＋CD8＋T cells between advanced lung cancer patients and normal controls. CD4^＋/ CD8^＋in patients with advanced small cell lung cancer were a lot less than those in normal controls. Conclusion： Both of the percentage of CD3^＋CD4^＋T cells in small cell and non-small cell advanced lung cancer patients were significantly decreased than that in normal controls. Patients who attacked with advanced lung cancer were severely injured in cellular immunity.