人黑色素瘤中肿瘤相关巨噬细胞的检测和临床意义

Clinical significance of tumor-associated macrophage in human skin melanoma

目的研究肿瘤相关巨噬细胞(tumor-associated macrophage,TAM)在人皮肤黑素瘤中的分布和数量及其临床意义。方法应用免疫组织化学方法检测74例人皮肤黑素瘤和30例色素痣CD68表达,分析CD68阳性的TAM与黑素瘤患者临床病理特征的关系。结果 TAM主要分布于黑色素瘤肿瘤的浸润前沿和肿瘤间隙,并且黑色素瘤组织中TAM计数显著多于色素痣(P<0.01);黑色素瘤TAM计数与与肿瘤的厚度、侵袭深度、淋巴结转移呈正相关(均P<0.05)。结论 TAM可能在皮肤恶性黑素瘤的生长、侵袭和转移过程中发挥了重要作用,是黑色素瘤恶性演进的一个潜在标志物。

Objective To investigate the role of tumor-associated macrophage （TAM）in human skin melanoma.Methods Immuno-histochemistry was used to detect the expression of CD68 in 90 skin melanoma and 30 nevus for labeling macrophage,and the clinico-pathological significance of TAMinfiltrating in melanoma was analyzed.Results Macrophage count was significantly higher in melano-ma than in nevus.Moreover,the count of macrophage was positively correlated to large tumor diameter,depth of tumor invasion and tumor lymph node metastasis in melanoma.Conclusions TAMmight play an important role in the growth,invasion and metastasis of skin melanoma,suggesting that count of TAMmight be a potential biomarker of melanoma progression.