灯盏花素注射液对脂多糖致大鼠急性肺损伤保护作用机制的研究

Protective effects of breviscapine injection on acute lung injury induced by lipopolysaccharides in rats

目的探讨灯盏花素注射液对脂多糖致大鼠急性肺损伤的保护作用。方法健康雄性SD大鼠30只随机分为对照组、脂多糖组(LPS组)和灯盏花素干预组(Bre组)各10只。对照组静脉注射生理盐水0.1 ml/kg;LPS组和Bre组静脉注射脂多糖5 mg/kg;Bre组在注射脂多糖前后30 min分别腹腔注射灯盏花素50 mg/kg。观察三组SD大鼠肺组织病理形态学改变及肺组织中超氧化物歧化酶活性(SOD)、丙二醛含量(MDA)及细胞间粘附分子-1(ICMA-1)的表达变化。结果与对照组比较,LPS组与Bre组SOD降低(P<0.05)、MDA增多(P<0.05)、ICMA-1表达增高(P<0.05);与LPS组比较,Bre组SOD升高(P<0.05)、MDA减少(P<0.05)、ICMA-1表达减少(P<0.05)。结论灯盏花素注射液可能通过提高机体抗氧化能力、降低细胞间粘附分子-1的表达来减轻脂多糖致大鼠急性肺损伤。

Objective To explore the protective effect of breviscapine（Bre） on acute lung injury induced by lipopolysaccharides（LPS） in rats and its possible mechanisms.Methods Thirty male Sprague-Dawley rats were randomly divided into three groups：Control group,LPS group,Breviscapine（Bre） group,each with 10 rats.Control group was injected by 0.1 ml/kg normal saline.LPS group and Breviscapine group was injected of LPS（5 mg/kg）.Additionally,Bre group received intraperitoneal injection of breviscapine（50 mg/kg） 30 minutes before LPS injection and 30 min after LPS injection.Morphological changes of lung tissues were observed under light microscope.The activity of superoxide dismutase（SOD）,the content of malondialdehyde（MDA） in lung tissues and the content of I intercellular adhesion molecule-1（CAM-1） in serum were detected in each group.Results Compared with Control group,the activity of SOD decreased（P〈0.05）,the content of MDA increased（P〈0.05）,and the content of ICAM-1increased（P〈0.05） in LPS group and Bre group.Compared with LPS group,the activity of SOD increased（P〈0.05）,the content of MDA decreased（P〈0.05） in lung tissues,and the content of ICAM-1 decreased（P〈0.05） in Bre group.Conclusion Breviscapine could reduce inflammation reaction in lung tissues during acute lung injury,which is probably through enhancing antioxidant activity of organism and decreasing the content of ICAM-1.