miR-30c对胃癌细胞株HGC-27侵袭转移能力的影响

Effects of miR-30c on the invasion and metastasis of HGC-27 cells in vitro

目的探讨miR-30c对胃癌细胞株HGC-27侵袭转移能力的影响。方法使用miR-30c mimics和inhibitor分别进行miR-30c的过表达和抑制,采用划痕实验和transwell小室实验分析癌细胞迁移和侵袭能力,Western blot检测上皮间质转化(EMT)相关蛋白E-cadherin、Vimentin和Fibronectin的表达差异。结果抑制miR-30c可促进胃癌细胞系HGC-27的侵袭(P<0.05),同时高表达miR-30c可抑制细胞侵袭(P<0.05)。抑制miR-30c后E-cadherin表达降低、Vimentin和Fibronectin表达升高,高表达miR-30c后E-cadherin的表达升高、Vimentin和Fibronectin表达下降。结论 miR-30c可能通过调节上皮间质转化(EMT)相关蛋白来影响胃癌的侵袭能力。

Objective To explore the effect of miR-30c on the invasion and metastasis of human gastric cancer cell line HGC-27 in vitro. Methods The overexpression and inhibition of miR-30c were induced with miR-30c mimics and miR-30c inhibitor. Wound healing assay and transwell invasion assay were conducted to assess the effects of migration and invasion capabilities of HGC-27 cells. The expression of epithelial mesenchymal transition （EMT） related proteins （E-cadherin, vimentin and fibronectin） was determined by Western blot. Results The inhibition of miR-30c promoted the migration and invasion capabilities of HGC-27 cells （ P 〈 0.05 ）. Meanwhile, the overexpression of miR-30c inhibited the migration and invasion capabilities of HGC-27 cells （P 〈 0.05 ）. The inhibition of miR-30c decreased the levels of E-cadherin expression and increased the levels of vimentin and fibronectin expression. The overexpression of miR-30c increased the levels of E-cadherin expression and decreased the levels of vimentin and fibronectin expression. Conclusion The results suggest that miR-30c may influence the invasion and metastasis capabilities of human gastric cancer via regulating EMT related proteins.