磷酸化蛋白EBP50通过降低ERK1/2活性抑制乳腺癌细胞MCF-7增殖能力

Phosphoprotein EBP50 suppresses proliferation of breast cancer by inhibiting activity of ERK1/2 in MCF-7 cell line

目的探讨磷酸化蛋白EBP50对乳腺癌细胞MCF-7细胞增殖的影响及其潜在机制研究。方法使用p GPU6/Neo载体构建稳定敲除EBP50表达的MCF-7细胞株,使用Western blot检测乳腺癌细胞中EBP50、c-myc、p-ERK1/2以及ERK1/2的表达,使用磺酰罗丹明B染色方法检测乳腺癌细胞增殖。结果使用p GPU6/Neo载体可以稳定地降低MCF-7细胞中EBP50的表达,敲除EBP50可以促进乳腺癌细胞增殖,同时促进c-myc的表达,上调ERK1/2的磷酸化水平,但不影响ERK1/2的表达。结论 EBP50可以通过抑制ERK1/2活性,抑制MCF-7乳腺癌细胞增殖能力。

Aim To investigate the relationship be-tween phosphoprotein EBP50 and the proliferation of breast cancer in MCF-7 cells. Methods The quali-fied recombinant plasmid sh-EBP50-pGPU6/Neo was transfected into MCF-7 cells with EBP50 knocking down. The expression of EBP50, c-myc, p-ERK1/2, and ERK1/2 was detected by Western blot. The prolif-eration ability of cells was detected by sulforhodamine B assay. Results The EBP50 knocking down plasmid was constructed successfully. MCF-7 cells with EBP50 knocking down had been established successfully. Knocking down of EBP50 increased the proliferation of MCF-7 significantly, and partially augmented the ex-pression of c-myc and phosphorylation of ERK1/2 . However, knocking down of EBP50 did not impact the expression of ERK1/2 . Conclusion EBP50 suppres-ses the proliferation of breast cancer cell through inhib-iting the activity of ERK1/2 in MCF-7 cell line.