CDK4和p16在喉鳞状细胞癌中的表达

Expression of CDK4 and p16 in laryngeal squamous cell carcinoma

目的:探讨CDK4、p16在喉鳞状细胞癌中的表达。方法:采用免疫组织化学SP法检测30例喉鳞状细胞癌组织和20例癌旁组织中CDK4和p16的表达情况,并分析二者分别与喉鳞状细胞癌临床分期和病理分级的关系。结果:在喉鳞状细胞癌中CDK4、p16的表达率分别为63.3%、46.7%,在癌旁组织中CDK4、p16的表达率分别为25%、90%。CDK4在喉鳞状细胞癌中的表达高于癌旁组织(P<0.05),与病理分级、临床分期均无显著相关性(P>0.05);p16在喉鳞状细胞癌中的表达低于癌旁组织(P<0.05),与病理分级有相关性(P<0.05),与临床分期无显著相关性(P>0.05);CDK4与p16的表达呈负相关(rs=-0.786,P<0.05)。结论:p16的低表达和CDK4的高表达可能在喉鳞状细胞癌的发生发展中起重要作用,且p16的低表达可能作为判断肿瘤恶性程度的指标。

Objective： To investigate the expression of CDK4 and p16 in laryngeal squamous cell carcinoma （LSCC）tissues. Method：The expressions of CDK4 and p16 in 30 cases of LSCC tissues and 20 cases of edge tissues were detected by immunohistochemical technology SP method,and discuss their correlation with clincial pathology and clinical stage of LSCC. Result：The positive rates of CDK4 and p16 were 63.3 % ,46.7% in LSCC tissues,and the positive rates were 25 %, 90% in edge tissues. The expression of CDK4 in LSCC tissues was significantly high- er than that in edge tissues（P〈0.05） ,which was not associated with the clincial pathology and clinical stage（P〉 0.05） ;The expression of p16 in LSCC tissues was significantly lower than that in edge tissues（P〈0.05） ,it was associated with the clincial pathology （P〈0.05）, but not associated with clinical stage（P〉0.05） ;there is a nega- tive correlation between CDK4 and p16 （r= - 0. 786, P〈0.05）. Conclusion： Low expression of p16 and high ex- pression of CDK4 may play an important role in the development of LSCC and the low expression of p16 in LSCC tissue could be used as important reference markers of malignant degree of tumour.