轴突导向因子Nein-1在先天性巨结肠中表达分布及其意义

The significance of the expression and distribution of axon Lidance factors Netrin-1 in Hirschsprung dis-ease

目的观察轴突导向因子Nein-1在先天性巨结肠（Hihpung’ diee，HD）患儿中正常段与痉挛段结肠中的表达情况，探讨其在HD发病机制中的作用。方法对36例HD患儿的正常段肠管（对照组）与痉挛段肠管（病变组），用免疫组织化学方法检测Nein-1在两组中的表达。结果在对照组（正常段）肠管中肌层（纵肌、环肌）、肌间神经节及黏膜层均见Nein-1表达，病变组（痉挛段）中肌层及黏膜层亦可见Nein-1表达，两组间Nein-1的表达强度差异无统计学意义（P〉0．05）。结论Nein-1在HD患儿正常段和痉挛段肠管的肌层、黏膜层表达无显著差异，可能说明Nein-1与HD的发生无明显相关性。

Objective To observe the expression and the distribution of Netrin1 in the spastic and nor mal segment of colon in patients with Hirschspmngs disease （HD） and investigate the relationship between Netrin1 and development of HD. Methods Colon specimens of 36 cases with HD were selected for this study. Normal segment of colon served as the control group, while the spastic segments of colon as the lesion group. Immunohistochemical staining was applied to detecte the expression and the distribution of Netrin1. Results Immunohistochemical staining showed that the expression of netrin1 was positive in the intestinal muscular lay ers （longitudinal muscle, circular muscles ）, myenteric ganglias and mueosal layers in the control group. However, the expression of Netrin1 was also positive in muscular layers and mucosal layer in the lesion group. There was no significant difference in the expression intensity of Netrin1 between the two groups （ P 〉 0. 05 ）. Conclusion The expression of Netrin1 in both the control group and the lesion group may indicate that netrin1 have no significant correlation with genesis of HD.