SDF-1/CXCR4轴在大鼠急性心肌梗死后BMSCs“归巢”中的作用

Role of SDF-1/CXCR4 in BMSCs "Homing" after Acute Myocardial Infarction

目的:观察祛瘀生新中药对急性心肌梗死后基质细胞衍生因子-1/配体CXC型趋化因子受体4(stromal cel1 derived factor-1/CXC chemokine receptor 4,SDF-1/CXCR4)轴的影响,探讨活血化瘀法对心肌梗死后骨髓间充质干细胞(bone mesenchymal stem cells,BMSCs)"归巢"的动员效应机制。方法:清洁级Wistar大鼠50只随机分为正常组、模型组、假手术组、中药组、西药组,每组10只。中药组大鼠术前3 d给药,1次·d-1。7 d后,检测外周血BMSCs含量,免疫组织化学检测各组大鼠心肌组织SDF-1、CXCR4表达水平及心肌c-kit细胞数,并观察心肌组织HE染色结果。结果:与假手术组比较,模型组心肌组织SDF-1、CXCR4表达均增加(P<0.05)。与模型组比较,中药组、西药组SDF-1、CXCR4表达水平及c-kit细胞数均增加,并能减轻心肌组织的病理损伤。结论:祛瘀生新法有动员BMSCs"归巢"的作用。

Objective：To observe the effect of removing stasis and promoting new Chinese medicine on stroma cell derived factor-1/CXC chemokine receptor 4（SDF-1/CXCR4）after acute myocardial infarction（AMI）in rats,and to investigate the mechanism of method of promoting blood circulation and removing blood-stasis on bone mesenchymal stem cells（ BMSCs）〝homing〝 after AMI. Methods：50 clean Wistar rats were randomly divided into five groups：normal group,model group,sham-operated group,Chinese medicine group, western medicine group. Each group was 10 rats. The Chinese medicine group was given administered 3 days before the operation,once a day. The rats were sacrificed 7 days later. Detected peripheral blood BMSCs and each rat myocardial tissue SDF-1 and CXCR4 expres- sion level and myocardial c-kit cell number by immunohistochemical and observed H-E staining results in colon. Results：The expres- sions of SDF-1 and CXCR4 in the model group were more than the sham group（P﹤0. 05）. Compared with the model group,The ex- pressions of SDF-1 and CXCR4 and c-kit cell number in the western medicine group and Chinese medicine group were all increased, and which can reduce the pathological damage of myocardial tissue. Conclusion：removing stasis and promoting new method can effec- tively mobilize the role of BMSCs〝Homing〝.