不稳定性心绞痛患者血管紧张素基因多态性与GLP-1的相关性研究

Relevant Research on Unstable Angina Angiotensin Gene Polymorphism Patients and GLP-1

目的:观察不稳定性心绞痛患者的血管紧张素转换酶基因插入/缺失多态性与胰高血糖素样肽1(glucagon-like peptide-1,GLP-1)的相关性,从而有效的指导中医的辨证施治。方法:应用聚合酶链反应(polymerase chain reaction,PCR)技术检测60例不稳定性心绞痛患者(观察组)和60例健康者(对照组)的血管紧张素基因插入(insertion,I)与缺失(deletion,D)多态性;采用双抗体一步夹心法酶联免疫吸附试验(double antibody-step sandwich enzyme-linked immunosorbent assay,ELISA)对胰高血糖素样肽1受体(glucagon-like peptide-1 receptor,GLP-1R)进行检测。结果:观察组血管紧张素DD基因型和血管紧张素D等位基因频率均高于对照组。GLP-1与不稳定性心绞痛血管紧张素DD基因型频率存在一定的联系。结论:DD基因型和D等位基因频率与不稳定性心绞痛的发生率呈正相关;不稳定性心绞痛血管紧张素DD基因型与GLP-1水平存在负相关性。

Objective：To observe patients with unstable angina angiotensin converting enzyme gene insertion / deletion polymorphism and glucagon-like peptide 1（GLP-1）correlation. Thus more effective guidance dialectical therapy of traditional Chinese medicine. Methods：Polymerase chain reaction（ PCR）technology to detect 60 cases of patients with unstable angina（ observation group）and 60 healthy sub- jects（ control group）of angiotensin（ ACE）gene insertion（ I）abulnd deletion（ D）and more normality;Adopted by double antibody-step sandwich enzyme-linked immunosorbent assay（ELISA）for glucagon-like peptide-1 receptor（GLP1R）were detected. Results：Unstable angina group compared with the control group,DD genotype and D allele frequencies were higher. Glucagon-like peptide 1has some links with unstable angina ACE-DD genotype frequencies. Conclusion：DD genotype and D allele frequency are positively correlated with un- stable angina,and there is a negative correlation between unstable angina ACE-DD genotype and glucagon-like peptide-1.