复方中草药提取物对小鼠机体抗氧化能力的影响

Effects of Herbal Medicament Extracts on Body's Antioxidant Ability in Mice

研究以乌梅、马齿苋等为主要成分的复方中草药提取物对小鼠机体抗氧化能力的影响。以昆明种雌性小鼠作为实验动物,试验设3个剂量组(100、200、400 mg/kg·bw)和1个溶剂对照组。灌胃28 d后,检测小鼠血清以及心、肝、脾、肾组织中的超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)的活性及丙二醛(MDA)的含量。复方中草药提取物在100、200、400 mg/kg·bw三个剂量水平上均可导致小鼠血清、心、肝、脾、肾组织中SOD和GSH-Px活性显著增强,降低小鼠上述组织中MDA含量,并呈现剂量反应关系。本研究中的复方中草药提取物可提高昆明种雌性小鼠机体的抗氧化能力,具有潜在的研究价值。

The research was aimed to study the in vivo antioxidant ability of herbal formulation which mainly consisted of Fructus Mume and Portulaca oleracea L. Female Kunming mice were selected as the experimental animals and three dose groups（ 100,200,400 mg / kg·bw） were set,meanwhile the distilled water was set as control. The activities of antioxidant enzymes（ superoxide dismutase and glutathione peroxidase） and malondialdehyde in serum,heart,liver,spleen and kidney were measured after the 28＇s oral administrations. Results showed that the three dose treatments of the herbal formulation extracts can significantly enhance the SOD（ superoxide dismutase） and GSH-Px（ glutathione peroxidase） activities and decrease the contents of MDA（ malondialdehyde） in serum,heart,liver,spleen and kidney of mice,and the antioxidant abilities were all showed in concentration-dependent manners. The herbal formulation can remarkable enhance the antioxidant ability in vivo and showed great potential for future research.