Mn^(2+)对仿刺参幼体的急性毒性试验

Acute Toxicity of Mn^(2+) to Larval Sea Cucumber Apostichpus japonicus

采用效应概率法,以"昏迷或死亡"和死亡为效应指标,研究了Mn2+对仿刺参五触手幼体、稚参及幼参的急性毒性。试验结果表明,Mn2+对五触手幼体24、48、96h"昏迷或死亡"的半效应质量浓度分别为220.5、197.8、173.7mg/L,推算的安全质量浓度为47.8mg/L;Mn2+对五触手幼体48、96h死亡的半效应质量浓度分别为207.6、177.3mg/L;Mn2+对稚参24、48、96h"昏迷或死亡"的半效应质量浓度分别为148.6、112.6、105.9mg/L,推算的安全质量浓度为19.4mg/L;Mn2+对稚参24、48、96h死亡的半效应质量浓度分别为189.4、125.9、105.9mg/L,推算的安全质量浓度为16.7mg/L;Mn2+对幼参24、48、96h"昏迷或死亡"的半效应质量浓度分别为315.4、253.3、149.0mg/L,推算的安全质量浓度为49.0mg/L;Mn2+对幼参48、96h死亡的半效应质量浓度为250.7、149.0mg/L。稚参对Mn2+毒性的承受力较弱,Mn2+对五触手幼体和幼参毒性较小,二者的安全质量浓度接近。

Acute toxicity of Mn2＋ to pentactula, and juvenile sea cucumber Apostichopus japonicus was studied using a probabilistie method and using＂coma and death＂ as an indicator. The coma median effect concentration （ECs0） of Mn2＋ to pentactula was found to be 220.5 mg/L for 24 h, 197.8 mg/L for 48 h and 173.7 mg/L for 96 h, with safe concentration （SC） of 47.8 rag/L; the lethal EC50 of Mn2＋ to pentactula was 207.6 for 48 h and 177.3 mg/L for 96 h. The coma EC50 of Mn2＋ to juveniles was 148.6 mg/L for 24 h, 112.6 mg/L for 48 h and 105.9 mg/L for 96 h, with SC of 19.4 mg/L; the lethal EC50 of Mn2＋ to juveniles was 189.4 for 24 h, 125.9 for 48 h and 105.9 mg/L for 96 h, with SCof 16.7 mg/L. The coma ECs0 of Mn2＋to large iuveniles was 315.4 for 24 h, 253.3 for 48 h and 149.0 mg/L for 96 h, with SC of 49.0 mg/L; the lethal EC50 of Mn2＋to large juveniles was 250.7 for 48 h and 149.0 mg/L for 96 h. The pentactula and large juvenile sea cucumber had stronger tolerance of Mn2＋ than juveniles did.