子痫前期产妇外周血中树突状细胞、Th1Th2细胞含量检测

Detection of peripheral blood dendritic cells,Th1Th2 cells content in patients with preeclampsia

目的:研究子痫前期产妇外周血中Th1Th2细胞含量以及树突状细胞表型。方法:将在本院确诊的60例子痫前期产妇纳入观察组,将同期进行产检的60例正常妊娠产妇纳入对照组,采用流式细胞仪检测外周血中Th1细胞、Th2细胞的含量以及树突状细胞表型,采用real-time PCR的方法检测外周血中肿瘤坏死因子-α(TNF-α)、干扰素-γ(IFN-γ)、白细胞介素-6(IL-6)、白细胞介素-10(IL-10)的mRNA含量,采用酶联免疫吸附法检测外周血中TNF-α、IFN-γ、IL-6、IL-10的蛋白含量。结果:观察组的Th1细胞含量、Th1/Th2细胞比例以及Th1类细胞因子TNF-α、IFN-γ的含量均高于对照组,Th2细胞含量以及Th2类细胞因子IL-6、IL-10的含量低于对照组,差异有统计学意义(P<0.05);树突状细胞表型:外周血中CD83+、CD80+以及CD86+阳性的树突状细胞比例均高于对照组,差异有统计学意义(P<0.05)。结论:子痫前期产妇外周血中Th1细胞功能增强、Th2细胞功能减弱,且成熟树突状细胞的数目增多。

Objective： To study the peripheral blood Th1Th2 cells content and dendritic cell phenotype. Methods： 60 cases of preeclampsia maternal diagnosed in our hospital were enrolled in observation group, 60 cases of healthy pregnant women received prenatal examination over the same period were enrolled in control group. Then Th1Th2 cells content and dendritic cell phenotype were detected by flow cytometry, peripheral blood TNF-α, IFN-r, IL-6, IL-10 mRNA content were detected by real-time PCR, TNF-α, IFN-r, IL-6, IL-10 protein content were detected by enzyme-linked immunosorbent adsorption method. Results： （1） Th1/Th2 cell function： Th1 cell content, Th1/Th2 proportion Th1 type cytokines TNF-α, IFN-r, IL-6, IL-10 content of observation group were higher than those of control group; （2） Dendritic cell phenotype： peripheral blood CD83＋, CD80＋ and CD86＋ positive dendritic cells proportion of observation group were higher than those of control group. Conclusion： In peripheral blood of preeclampsia women, Th1 cells function increase, Th2 cells function decrease and mature dendriticcells content increase.