女性宫颈细胞中HPV感染基因型别的分析

Analysis of HPV infection genotype spectrum in female cervical cells

目的探讨女性宫颈细胞中39种人乳头瘤病毒(human papillomavirus,HPV)感染基因型别的分布情况及其临床意义。方法从434例女性宫颈细胞标本中提取39种HPV DNA,采用基因扩增结合基因芯片技术对其细胞进行39种HPV基因型别的检测,并对其患者进行临床资料分析。结果 434例女性宫颈细胞标本中检出HPV阳性者175例,总的HPV感染率40.32%(175/434)。其中单一HPV感染105例,阳性检出率为24.19%(105/434),多重HPV感染70例,阳性检出率为16.13%(70/434)。单一HPV感染中HPV18型为31例,阳性检出率为17.71%(31/175),是最主要的感染型别;其次是HPV16型,共12例,其阳性检出率为6.86%(12/175),HPV52型,为11例,其阳性检出率为6.29%(11/175)。多重HPV感染中,HPV6+54型、HPV18+52型和HPV51+68型各2例,各占多重感染的2.86%(2/70),它们是多重HPV感染的最主要型别。结论 HPV16型、18型、52型、6+54型、18+52型和51+68型感染是女性宫颈细胞的主要感染类型。基因扩增结合基因芯片技术是一种适合临床开展HPV分型诊断和行HPV感染的分子流行病学研究的方法。随着HPV检测型别的增加,HPV感染率也随之增加,其基因组合型别更趋多样化。

Objective To investigate the distribution of 39 kinds of human papillomavirus（HPV）infection genotypes in female cervical cells and its clinical significance.Methods 39 types of HPV DNA were extracted from 434 samples of female cervical cells. The gene amplification combined with the gene chip technique was adopted to detect 39 kinds of HPV genotype.And the clinical da-ta of the patients were analyzed.Results Among 434 samples of female cervical cell,175 cases were HPV positive,the total HPV infection rate was 40.32%（175/434）.Among them,105 cases were the single type HPV infection with the positive detection rate of 24.19%（105/434）and 70 cases were the multiple types HPV infection with the positive detection rate of 16.13%（70/434）.Among single type HPV infection,31 cases were the HPV18 infection with the positive detection rate of 17.71%（31/175）,which was the main HPV infection type;followed by HPV16 in 12 cases with the positive detection rate of 6.86%（12/175）and HPV52 in 11 cases with the positive detection rate of 6.29%（11/175）.Among the multi-type HPV infection,each 2 cases were HPV 6＋54,HPV 18＋52,HPV 51＋68 infection respectively,each accounted for 2.86% of the multi-type HPV infection,which were the main infection types.Conclusion HPV 16,18,52 and HPV 6＋54,HPV 18 ＋52 and HPV 51 ＋68 are the main HPV infection genotypes of fe-male cervical cells.The gene amplification combined with the gene chips technique is a method suitable for clinically conducting the HPV genotyping diagnosis and the molecular epidemiologic research of HPV infection.Along with the increase of detected HPV genotypes,the HPV infection rate is also increased,its genotypes combinations trend towards diversification.