N、P、K异质含量拟南芥试管苗的表型适应特性

Phenotypic adaptive properties of Arabidopsis thaliana test-tube plantlets inoculated in the medium with heterogeneous content of N,P and K

以模式种拟南芥为试验材料,有别于自然环境下生长的拟南芥,借助于组织培养方法利用均匀设计对培养基中KNO3、NH4NO3、KH2PO4设置处理,探讨试管环境下拟南芥生长发育特性及对营养离子需求吸收特点,结果显示拟南芥本性地通过加强莲座叶与茎生叶的功能以增加生殖生长期的分枝数与苔高;生殖生长期影响试管苗茎生叶数、苔高的离子种类增多,离子的交互作用更加复杂。通过分析模式植物对N、P、K异质含量培养基的表型适应特点可推论同一植物在不同时期使用单一培养基是不合理的。

Characteristics of growth and development along with the specialty and the demand of absorbing nutritional ions of Arabidopsis thaliana in vitro were discussed by setting mode plant A .thaliana as the experimental material, unlike natural growth of Arabidopsis and by setting different levels of KNO3 ,NH4 NO3 and KH2 PO4 of treatment medium with in virtue of tissue culture technology and uniform design.The results showed that A .thaliana plantlets would reinforce the physiological function of rosette leaf and cauline leaf to enlarge the branches and the height of trunk;In reproductive phase,ion species which affect the number of cauline leaf and the height of trunk increased,the interaction of mineral ions was more complicated.Through analyzing photo-adaptive properties of Arabidopsis thali-ana test-tube plantlets inoculated on the medium with heterogeneous content of N,P and K,it could be deduced that it was unreasonable to use unitary medium aim at certain plant into different growth period.