两种生物碱联合氨苄西林对表皮葡萄球菌抗菌作用研究

Research on Antibiotic Action of Two Kinds of Alkaloids Combining with Ampicillin on Staphylococcus Epidermidis

检测盐酸小檗碱和苦参碱联合氨苄西林对表皮葡萄球菌的抗菌作用.利用二倍稀释法、微量棋盘稀释法、时间-杀菌曲线评估法对8株临床分离的表皮葡萄球菌进行药物敏感性测定.结果表明，盐酸小檗碱、苦参碱、氨苄西林单药对表皮葡萄球菌的最小抑菌质量浓度分别为0.8-3.1μg/mL、12.5 mg/mL 和0.5-16.0μg/mL.盐酸小檗碱联合氨苄西林作用于8株分离株后，盐酸小檗碱和氨苄西林的最小抑菌质量浓度分别下降了88%-94%和75%，抑菌浓度指数为0.187-0.498；苦参碱联合氨苄西林作用于8株分离株后，苦参碱和氨苄西林的最小抑菌质量浓度分别下降了80%和75%-94%，抑菌质量浓度指数为0.188-0.450.2种生物碱联合氨苄西林作用于表皮葡萄球菌的时间-杀菌曲线显示，菌落减少量大于等于2 lg N.盐酸小檗碱、苦参碱与氨苄西林联合作用表皮葡萄球菌均具有抗菌协同作用.

This article is about testing the antibiotic action of berberine hydrochloride and matrine ’s combining with ampicilin on staphylococcus epidermidis.8 clinical isolated staphylococcus epidermidis were tested for drug susceptibility with two-fold dilution method,micro-chessboard assay method and time-sterilization curve evaluation method.The result shows that the minimum bacteriostasis concentration of berberine hydrochloride, sophocarpidine and ampicillin to staphylococcus epidermidis were 0.8-3.1μg/mL,12.5 mg/mL and 0.5-16.0 μg/mL respectively. After the action of berberine hydrochloride’s combining with ampicillin on 8 isolated staphylococcus, the minimum bacteriostasis concentration of berberine hydrochloride and ampicillin were down,88%-94% and 75% respectively,and fractional inhibitory concentration index was 0.187-0.498;The minimum bacteriostasis concentration of sophocarpidine and ampicillin declined,80% and 75%-94% respectively after the action of sophocarpidine combining with ampicillin on 8 isolated staphylococcus,and the bacteriostatic concentration index was 0.188-0.450.The time-sterilization curve of actions of two kinds of alkaloid’s combining with ampicillin on staphylococcus epidermidis shows that the decrease amount of bacterial colony is larger than 2lg N.The combination of both berberine hydrochloride and sophocarpidine with ampicillin is antibacterial synergy when acting on staphylococcus epidermidis.