益母草化学成分研究Ⅲ

Study on chemical constituents of Leonurus japonicus Houtt. Ⅲ

[目的]继续对益母草(Leonurus japonicus Houtt.)的化学成分进行研究,为进一步开发利用该植物资源提供依据。[方法]采用正相硅胶、反相ODS、Sephadex LH-20等柱色谱及高效液相色谱法等方法进行分离纯化,并通过理化性质及光谱分析鉴定化合物的结构。[结果]从益母草体积分数为50%的乙醇提取物中分离鉴定了7个化合物,分别为(E)-4-hydroxy-dodec-2-enedioic acid(1)、益母草碱(2)、水苏碱(3)、次黄苷(4)、鸟苷(5)、左旋色氨酸(6)、苯丙氨酸(7)。[结论]1、4、5、7为从益母草种中首次分离得到的化合物。

[Objective] To investigate the chemical components of the herb Leonurus japonicus Houtt. Ⅲ.[Methods] The chemical components were obtained by chromatographies, such as silica gel, ODS, Sephadex LH-20 and HPLC. They were identified by the chemical and physical methods, especially spectral analysis. [Results] Seven compounds were obtained and identified as（E）-4-hydroxy-dodec-2-enedioic acid（1）, leonurine（2）, stachydrine（3）,hypoxanthine-9-β-D-ribofuranoside（4）, guanosine（5）, L-tryptophan（6）, and phenylalanine（7）, respectively.[Conclusion] Compounds 1, 4, 5, and 7 are the obtained species firstly.