芎芷地龙汤对偏头痛风热证血管活性物质的影响

Effect of Xiongzhi Dilong Decoction on Vasoactive Substance in Migraine with Wind heat Syndrome

目的探讨芎芷地龙汤对偏头痛风热证血管活性物质的影响。方法使用热风及硝酸甘油协同诱导建立大鼠偏头痛风热证模型,观察2 h内搔头次数,用放免法测定血浆中内皮素(ET)、降钙素基因相关肽(CGRP)、血栓素A2(TXA2)、前列环素(PGI2)含量。方法偏头痛模型组血浆TXB2、ET、CGRP含量升高,6 Keto PGF1α含量降低,与正常组、风热组相比差异有统计学意义(P<0.05);偏头痛风热证组血浆TXB2、ET、CGRP含量继续升高,6 Keto PGF1α含量继续降低,与正常组、偏头痛模型组比较有统计学意义(P<0.05或P<0.01);经过治疗后血浆TXB2、ET、CGRP、6 Keto PGF1α含量回复,与偏头痛风热证组及偏头痛模型组相比差异有统计学意义(P<0.05)。结论风热对正常机体无影响,而对硝酸甘油致偏头痛模型病理状态有显著影响,偏头痛模型受风热外袭后血管活性物质严重失衡,芎芷地龙汤能明显改善偏头痛风热证动物模型血管活性物质失衡状态。

Objective To study the effect of Xiongzhi Dilong decoction（XDD）on vasoactive substance in migraine with wind heat syndrome.Methods To establish migraine with wind heat syndrome model by combining exterior pathogen （hot wind）and GTN ad-ministration,examine the frequency of scratching head within 2 h,and survey the content of thromboxane B2 （TXB2 ）,6 Keto PGF1α,calci-tonin gene related peptide（CGRP）,endothelin（ET）in blood plasma by radioimmunoassay technology.Results The content of TXB2 ,ET and CGRP in plasma of migraine model was significantly higher than normal group and wind heat group,and the content of 6 Keto PGF1αwas significantly lower than normal group and wind heat group.The content of TXB2 ,ET and CGRP in plasma of migraine with wind heat syndrome moldel persistently increased,and The content of 6 Keto PGF1αpersistently decreased,which is significant difference compared to normal group（P〈0.01）,and is significant difference compared to migraine model group（P〈0.05）. The content of TXB2 ,ET,CGRP and 6 Keto PGF1αin plasma of Chinese medicine treatment group is recovered,which was signifi-cant difference compared to migraine with wind heat syndrome model and migraine model group（P〈0.05）.Conclusion There was no significant effect on normal organism which sufferd from wind heat,But there was significant effect on migraine model induced by Nitroglycerin,The vasoactive substance of Migraine model which was invaded by wind and heat was disbalance severely.XDD could ameliorate remarkably disbalance state of vasoactive substance in wind heat syndrome migraine model.