福州动物园工作人员及普通人员高水平耐庆大霉素肠球菌耐药表型检测

Detection of Resistant Phenotype of High-Level Gentamicin-Resistant Enterococcus from Fuzhou Zoo Staff Member and The General Population

为了解福州动物园饲养员和兽医以及普通人群的细菌耐药现状,选用肠球菌为指示菌,采用琼脂平板筛选法(ADSP法)筛选高水平耐庆大霉素肠球菌(HLGRE);根据美国临床实验室标准化协会(CLSI)推荐的微量肉汤稀释法测定耐药菌株对6种抗菌药物的耐药性;用PCR法对分离出的不同来源的高水平耐氨基糖苷类肠球菌进行菌种的鉴定。从所有37个样本中共筛选出15株HLGRE。其中动物园饲养员及兽医的分离率为62.50%,而普通人群的分离率为23.81%,差异显著。分离到的肠球菌中,以粪肠球菌为主要,根据药敏实验结果显示,不同人群的H1GRE均以红霉素和土霉素的耐药率最高,均达到100%;

We studied bacterial resistance in the keepers and veterinarians at Fuzhou Zoo and the general population. Enterococcus was chosen as the indicator and the ADSP method was used to screen high-level gentamicin-resistant Enterococus（HLGRE）. A micro-broth-dilution method recommended by CLSI was used to test the drug resistance of Enterococcus to 6 types of antimicrobial. HLGRE from different sources was confirmed by PCR. There were 15 strains of HLGRE isolated from a total of 37 human source samples. Zoo personnel showed a significantly higher rate of isolation of HLGRE（62.50% ）than did the general population（23. 81% ）. HLGRE was E. faecalis based. Drug sensitivity tests indicated that HLGRE from different sources showed the highest drug resistance to ERY and OTC（100% ）, and lowest drug resistant to AMP and VAN.