恩施续断中含硒化合物体外抗氧化活性分析

Study on Combined Forms of Selenium and Antioxidant Activity in Enshi Dipsacus

以恩施续断为原料,用蒸馏水、0.5 mol/L Na Cl、75%乙醇、0.1 mol/L Nao H四种溶剂对续断中不同种类硒蛋白进行提取.用热水浸提法提取硒多糖,用原子荧光法测定不同提取物中硒元素的含量,分析了续断中硒的赋存形态.并采用邻苯三酚自氧化法测定续断不同提取物清除超氧阴离子(O2-·)的能力,用Fenton法测定其清除羟自由基(·OH)的能力,探究了含硒化合物的抗氧化活性.结果表明:续断中硒的总含量为1.538μg/g,蛋白质、多糖中都赋存一定量的硒;蛋白硒是续断中硒的主要赋存形态.四种溶剂提取物中碱溶性硒蛋白的含量最高,它的含量可达总可溶性蛋白的57.28%.按照相同质量样品中结合硒量的多少,可以得出含硒量:总蛋白硒>多糖硒;醇溶性硒蛋白>盐溶性硒蛋白>碱溶性硒蛋白>水溶性硒蛋白.恩施续断中各种含硒化合物都有一定的清除超氧阴离子和羟自由基的能力,且不同溶剂提取物的清除率都不同.其中醇溶性提取物清除超氧自由基的能力最好,碱溶性提取物清除超氧自由基的能力最弱;硒多糖清除羟自由基的能力最强.

Taking Enshi Dipsacus as raw material, different kinds of selenoproteins were extracted with distilled water,0.5 mol/L NaCl solution,75%ethanol and 0.1 mol/L NaoH.The hot water extraction meth-od was used to extract selenium polysaccharide,the atomic fluorescence spectrometry was used to deter-mine the selenium content in different extracts,and the forms of ocuorrence of selenium in Dipsacus were analyzed.And pyrogallol Autoxidation method was used to determine the ability of scavenging superoxide anion of different extracts on Dipsacus, using fetion eaction determination of the ability of scavenging of hydroxyl radicals,Explores the antioxidant activity of organoselenium compounds.The results showed that Total content of selenium in Enshi Dipsacus is 1.538μg/g,Occurrence in a certain amount of selenium both proteins,and polysaccharides,but Selenium-protein is the mainly forms of selenium in Dipsacus.The highest concentrations of four kinds of solvent extraction is alkali-soluble selenium protein,whose content of total soluble protein is 57.28%.According to the same quality samples in the combined amount of sele-nium,selenium content of order can be calculated as：Selenoprotein 〉polysaccharides containing seleni-um;Alcohol-soluble selenoprotein〉salt-soluble selenoprotein〉alkali soluble selenoprotein〉water solu-ble selenoprotein.Diffient kind of selenium-containing compounds of Enshi Dipsacus that have a certain degree of superoxide anion and hydroxyl free radical scavenging ability.And of different solvent extracts from different clearance rate, Alcohol-soluble extracts of which rate is greater than oxygen free radical＆nbsp;scavenging ability of the best.Alkali-soluble extracts of the weakest ultra oxygen free radical scavenging capacity,selenium polysaccharide of the greatest hydroxyl radical scavenging capacity.