基于LSU和ITS的青藏高原黄绿卷毛菇种群遗传多样性分析

The genetic structure analysis of Floccularia luteovirens using LUS and ITS assay

为研究黄绿卷毛菇群体遗传结构,以青海、西藏、四川3个省份的10个地理群共91个样本黄绿卷毛菇为实验材料,采用分子生物学方法测定和分析所有样本的LSU和ITS序列。结果显示:1 995bp的LSU和ITS基因序列共有8个变异位点,得到20个基因型。单倍型多样性指数(Hd)和核苷酸多样性指数(pi)分别为0.538±0.026和0.00029±0.00002;青海玉树和青海兴海的遗传多样性指数最高。分子变异分析结果表明黄绿卷毛菇群体遗传变异主要来自种群内(75.70%),种群间遗传分化较大。系统进化树结果也表明,10个地理群分成2个支系,黄绿卷毛菇群体存在明显的地理结构。中性检测结果进一步证实,黄绿卷毛菇群体有着复杂的群体历史,曾发生过种群数量扩张,近期种群数量正在衰减。

For the study of Floccularia luteovirens population genetic structure, ITS and LSU sequences of 91 samples of Floccularia luteovirens belonging to 10 geographic group from Qinghai, T ibet and Sichuan were analyzed. Eight variable sites existed among the aligned sequences of 1 995 bp, and 20 genotypes were found. The average haplotype diversity（Hd） was 0.538±0.026, while the nucleotide diversity（pi） was 0.00029±0.00002. Analysis results of molecular variance showed that the majority of genetic variation of the Floccularia luteovirens groups occurred within populations（75.70%）, and the genetic difference among populations was greater. Phylogenetic tree also showed that 10 populations of Floccularia luteovirens were divided into two branches, illustrating that Floccularia luteovirens had obvious geographical structure. The neutrality test resultsfurther confirmed that the Floccularia luteovirens groups had relatively complicated demographic history, undergoing a long process from population expansion to recent populations decline.