灰葡萄孢交配型基因的分析与检测

Analysis and molecular detection of Botrytis cinerea mating type genes

通过生物信息对灰葡萄孢的MAT1‐1‐1与MAT1‐2‐1氨基酸序列进行了系统进化与结构域保守氨基酸分析,表明灰葡萄孢的交配型蛋白与核盘菌的亲源关系最近,结构域氨基酸比对结果表明该基因具有保守氨基酸的一致性与部分氨基酸的相似性。应用PCR技术检测灰葡萄孢交配型基因MAT1‐1‐1与MAT1‐2‐1,结果表明各种植区交配型菌株所占比例有较大的差异,多数种植区灰葡萄孢同时存在MAT1‐1与MAT1‐2两种交配类型,快速检测灰葡萄孢的交配型等位基因对于灰葡萄孢种群结构分析非常有意义。

The phyletic evolution and conserved domain of amino acid sequence of grey mold Botrytis cinerea, MAT1‐1‐1 and MAT1‐2‐1 were analyzed. The results showed that the genetic relationship of B. cinerea mating type protein MAT1‐1‐1 and MAT1‐2‐1 was close to that of Sclerotinia sclerotiorum. The amino acid sequence alignment of mating type protein domains showed that the genes had conserved amino acid identity and partial amino acid similarity. The mating type genes, MAT1‐1‐1 and MAT1‐2‐1, were detected by PCR technique and the results showed that the mating type distribution ratio of the strains in different fields had great difference. In most planting regions both mating types were detected. Quick detection of mating type genes from grey mold isolates is very important for the analysis of population structures based on mating type patterns.