响应面优化裂褶菌多糖酶解工艺及抑菌研究

Response surface optimization of enzymatic hydrolysis and anti‐microbial activities of schizophyllan polysaccharose

探索了裂褶菌多糖酶解法提取工艺及酶解产物抑菌的作用。分别从p H、酶解时间、酶解温度和加酶量4个因素考察了对裂褶菌多糖提取率的影响,通过响应面分析进行裂褶菌多糖提取工艺条件的优化。结果表明,在液体果胶酶p H为5.5、酶解时间1.2h、酶解温度50℃、加酶量1%时裂褶菌多糖提取率达54.76%;以大肠杆菌、假单胞菌为供试菌株,通过抑制率来反映其抑菌效果,结果表明裂褶菌多糖对大肠杆菌、假单胞菌有一定的抑制作用。

The extraction technology and anti‐microbial activities of schizophyllan polysaccharose were studied by enzymatic technology. The effects of pH, enzymolysis duration, enzymolysis temperature and additive amount of enzyme on schizophyllan extraction rate were investigated. Based on single factor experiments, response surface methodology was used to optimize the process parameters. Results showed that under the conditions of liquid pectinase＇s pH value of 5.5, enzymolysis duration of 1.2h, enzymolysis temperature of 50°C and enzyme additive amount of 1%, the schizophyllan polysaccharose extraction rate was 54.76%. Schizophyllan had inhibiting effect to Escherichia coli and Pseudomonas sp. proliferation.