肝癌组织中p-gp170、GST-π、TopoⅡ蛋白的表达

Expressions of p-gp170,GST-π,TopoⅡ in the Hepatocellular Carcinoma

目的探讨p-gp170、GST-π和TopoⅡ在肝癌组织中的表达及临床意义。方法采取免疫组化法检测35例肝癌组织、20例癌旁组织和10例肝硬化组织中p-gp170、GST-π和TopoⅡ蛋白的表达,并对其与组织病理参数及生存率的关系进行分析。结果肝癌组织中的p-gp170、GST-π的阳性表达率均显著高于癌旁组织及肝硬化组织(P<0.05)。肝癌组织中TopoⅡ蛋白的表达与癌栓关系密切(P<0.05)。肝癌组织中p-gp170+GST-π的阳性表达率高于p-gp170+GST-π+TopoⅡ、p-gp170+TopoⅡ、GST-π+TopoⅡ的阳性表达率(P<0.05)。TopoⅡ阳性表达者的2 a生存率低于阴性表达者(P<0.05)。结论 p-gp170、GST-π在肝癌组织中的高表达,可作为肝癌组织恶变的标志物;TopoⅡ与肝癌的浸润转移及生存关系密切。

Objective To investigate the expressions and clinical significance of p-gp170,GST-πand TopoⅡin the hepatocellular carcinoma. Methods Immunohistochemical method was used to detect the expressions of p-gp170,GST-π and TopoⅡ in the 35 cases of hepatocellular carcinoma,the 20 cases of paraneoplastic tissues form hepatocellular carcinoma and the 10 cases of hepatic cirrhosis. The relationship with clinicopathologic parameters and survival rate were comprehensively analyzed. Results The positive expression rate of p-gp170,GST-π in the hepatocellular carcinoma were higher than those in the 20 cases of paraneoplastic tissues and the 10 cases of hepatic cirrhosis（P〈0. 05）. The expression of TopoⅡin the hepatocellular carcinoma was related with cancer embolus（P〈0. 05）. In the hepatocellular carcinoma,the positive expression rate of p-gp170＋GST-πwas higher than those of the p-gp170＋GST-π＋TopoⅡ,the p-gp170＋TopoⅡ,the GST-π＋TopoⅡ（P〈0. 05）. The 2-year survival rate of the hepatocellular carcinoma with TopoⅡ positive expression was lower than that of the hepatocellular carcinoma with TopoⅡ negative expression（P〈0. 05）. Conclusion p-gp170,GST-π are higher expressions in the hepato-cellular carcinoma,and can be the marker of hepatocellular carcinoma;TopoⅡ may be relative to imbibition and survival of the hepatocellular carcinoma.