天麻成分对羟基苯甲醇抗实验性脑血栓形成及抗炎作用研究

Anti-thrombotic and Anti-inflammatory Effects of 4-Hydroxybenzyl Alcohol from Gastrodia elata

目的观察天麻成分对羟基苯甲醇抗脑血栓形成及抗炎作用.方法本实验采用经大鼠一侧颈内动脉注射花生四烯酸诱发同侧大脑半球脑血栓形成方法评价天麻成分对羟基苯甲醇的抗实验性脑血栓形成作用;采用二甲苯诱导小鼠耳廓肿胀模型、花生四烯酸、角叉菜胶诱导大鼠足趾肿胀反应模型考察了天麻成分对羟基苯甲醇的抗炎作用.结果天麻成分对羟基苯甲醇20 mg/kg和15 mg/kg组均能够降低花生四烯酸注射侧大脑伊文思蓝吸光度与脑重的比值(P<0.01);天麻成分对羟基苯甲醇30 mg/kg、20 mg/kg和15 mg/kg组均能够显著抑制二甲苯诱导小鼠耳廓肿胀,抑制率分别为60.01%、52.72%、45%(P<0.01);天麻成分对羟基苯甲醇20 mg/kg组能够抑制大鼠足趾注射角叉菜胶诱导的足趾肿胀反应,抑制率为42.56%(P<0.01),对大鼠足趾注射花生四烯酸诱导的足趾肿胀反应无明显影响.结论天麻成分对羟基苯甲醇具有显著的抑制实验性脑血栓形成及抗炎作用,其抗炎作用与抑制花生四烯酸环氧合酶代谢途径有关.

Objective The study was aimed to study the anti-thrombotic and anti-inflammatory effects of 4-hydroxybenzyl alcohol from Gastrodia clara. Methods Cerebral thrombosis caused by injection of arachidonie acid （AA） via intracarotid artery method was used to evaluate the anti-thrombotic effect of 4-hydroxybenzyl alcohol. Two methods were used to evaluate anti-inflammatory effect of 4-hydroxybenzyl alcohol, including mouse ear edema induced by xylene and rat metatarsal swelling induced by a single subcutaneous injection of AA or carrageenan. Results The results showed that 4-hydroxybenzyl alcohol markedly reduced the ratio of Evans blue and infracted hemisphere weight in 20 mg/kg and 15 mg/kg （P〈0.01） . And it showed significantly inhibitory effect on mouse ear edema induced by xylene （P〈0.01）, the inhibitory rates were 60.01% , 52.72% and 45% is 30 mg/kg, 20 mg/kg and 15 mg/kg, respectiuely. And it showed inhibitory effect of rat metatarsal swelling reaction induced by carrageenan, the inhibitory rate was 42.56% in 20 mg/kg （P〈O.01） , and it had no effect on Toes swelling reaction induced by AA. Conclusion 4-Hydroxybenzyl alcohol, a component of G. clara, had significant inhibition of experimental cerebral thrombosis and anti-inflammatory effect by inhibiting arachidonic acid cyclooxygenase metabolic pathways.