水提紫甘薯色素废渣花色苷纯化工艺研究

Purification Process of Anthocyanins from Ipamoea batatas Residue after Water Extraction for Pigment

目的在前期对水提紫甘薯色素废渣花色苷提取研究基础上,进一步深入研究花色苷纯化工艺,旨在为水提紫甘薯色素废渣的综合开发利用提供理论基础和参考依据.方法通过静态吸附、解吸和动态吸附、解吸等试验来考察AB-8大孔树脂的纯化性能,对水提紫甘薯色素废渣花色苷粗提液进行纯化.结果 AB-8大孔树脂对紫甘薯废渣花色苷具有较强的吸附能力,吸附率达81.47%;溶液p H为1.5时,对花色苷的吸附效果最好;解吸液以2BV浓度为80%的乙醇水溶液解吸效果最好,解吸率达65.69%;解吸流速以1 m L/min效果最好.纯化后的花色苷解吸液,经旋转蒸发浓缩,浓缩液在-80℃超低温冰箱冷冻后进行冷冻干燥得到冻干粉.经AB-8大孔吸附树脂二次纯化后,水提紫甘薯色素废渣花色苷纯度达91.37%.结论采用AB-8大孔吸附树脂纯化水提紫甘薯色素废渣花色苷所得工艺具有较好纯化效果,方法简便可行,经二次纯化后的水提紫甘薯色素废渣花色苷纯品可应用于后期的生物活性研究及实际生产.

Objective The study was aimed to further investigate the purification process of anthocyanins from Ipamoea batatas residue after water extraction for pigment, in order to provide the theoretical basis and reference for the comprehensive development and utilization of I. batatas residue. Methods The depurated performance of AB-8 macroporous adsorption resin was investigated through static adsorption and desorption, dynamic adsorption and desorption experiments. Then the crude extraction of anthocyanins from I. batatas residue was purified. Results The AB-8 macroporous adsorption resin had strong adsorption capacity for anthocyanins from crude extraction of I. batatas residue, with the adsorption rate of 81.47%. The solution with pH 1.5 had the best adsorption effect on anthocyanin. The best desorption rate of anthocyanins was 65.69% when 2BV of 80% ethanol was used as eluent with flow rate of lmL/min. After evaporation and concentration, the concentrated solution of anthocyanins was froze and dried in ultra low temperature freezer of -80℃ to obtain powder. The purity of anthocyanins from I. batatas residue was 91.37% after purification two times on AB-8 macroporous adsorption resin. Conclusion The purification process of anthocyanins from I. batatas residue after water extraction for pigment with AB-8 macroporous adsorption resin has a good purification effect. The method is simple and feasible. The pure anthocyanin products from residue could be applied in biological activity research and practical production.