基于NaYF_4∶Yb^(3+),Er^(3+)上转换发光纳米晶的复合微球组装及其光动力活性

Composite Microspheres Assembly and Photosensitive Activity Research Based on NaYF_4:Yb^(3+),Er^(3+) Upconverting Nanoparticles

采用微乳液法,以NaYF4∶Yb3+,Er3+纳米晶为发光基元,肽菁锌(Zn Pc)光敏分子与十八碳烯-马来酸酐共聚物(PMAO)为功能分子,一步组装获得了NaYF4-Zn Pc-PMAO复合微球,此微球同时具备成像与光动力活性功能,NaYF4可作为低生物背景的荧光成像剂,同时其上转换发光可以敏化Zn Pc用于光动力活性研究,PMAO分子经过简单的水解反应即可实现表面羧基功能化。TEM,Zeta电位与PL测试证实了微球的结构与性能。利用荧光共聚焦成像技术实现了对Hela细胞的发光成像;进一步通过单线态氧监测及980 nm光照下的MTT法细胞活性测试表明微球具有光动力活性功能。

A multifunctional composite microspheres were prepared based on NaYF4∶Yb^3＋,Er^3＋ upconversion nanoparticles（UCNPs）,zinc phthalocyanine（Zn Pc） and poly（maleic anhydride-alt-1-Octadecene）（PMAO） via a one step self-assembly process in microemulsion droplets.The UCNPs as fluorescence imaging agent can be used for biological imaging,and can also use the up-convering luminescence sensitization photosensitive molecules for the research of photosensitive activity.PMAO molecules provide anhydride functional groups for immobilization of biomolecules.The morphologies and property were characterized by transition electron microscopy（TEM）,Zeta potential and photoluminescence（PL） spectroscopy.The fluorescence confocal cell imaging confirms that the NaYF4-Zn Pc-PMAO are suitable for potential biological labeling.Photodynamic cancer cell killing ability was evaluated using a MTT assay with Hela cells under different time irradiations.The results indicating that the NaYF4-Zn Pc-PMAO composite microspheres have highly killing ability to Hela cells under 980 nm light irradiation.