人结直肠癌细胞株研究及使用现状

Current study and application of human colorectal cancer cell lines

结直肠癌细胞株是研究结直肠肿瘤癌变、浸润转移机制、探寻新的治疗药物及策略的理想模型。然而，目前存在结直肠癌细胞株数目众多、质量参差不齐以及研究者对其生物学特性重视不足等众多问题，导致结直肠癌研究中对细胞株的选择及使用不规范，影响实验结果的准确性，甚至可能得出错误结论。本文对迄今已建立的人结直肠癌细胞株进行整理分类，从其建立和应用、分类与选择、使用现状与存在问题3方面进行总结。

Colorectal cancer（CRC） cell lines are ideal in vitro models for colorectal cancer study. Although the number of colorectal cell lines increases hence provides diversified choices for research, several problems occur including the uneven quality control, insufficient attention to the origin and biological characteristics of CRC cell lines , resulting in inappropriate selection of CRC cell lines for study. In this paper, we classify the current CRC cell lines for study, review the biological characteristics, current problems of experiment choices, and discuss the exact choice of cell lines for CRC study.