辛伐他汀上调肾小管上皮细胞ADAMTS13 mRNA表达

Simvastatin upregulates ADAMTS13 mRNA expression in human renal tubular epithelial cell line HK-2

目的观察不同细胞因子对近端肾小管上皮细胞株(HK-2)血管性血友病因子裂解酶(ADAMTS13)的影响及辛伐他汀干预结果。方法炎症因子按浓度分组:TNF-α0、0.1、1、10ng/ml分为A0-A3组;IL-6 0、1、10、100ng/ml分为B0-B3组;IL-4 0、1、5、10ng/ml分为C0-C3组。辛伐他汀0、0.1、1、10μmol/L分为D0-D3组。两者共培养分成不同浓度辛伐他汀+TNF-α10ng/ml(E0-E3)组,不同浓度辛伐他汀+IL-6 100ng/ml(F0-F3)组和不同浓度辛伐他汀+IL-4 10ng/ml(G0-G3)组。孵育HK-2 24h,用实时定量PCR法和Western blot法检测ADAMTS13的mRNA及蛋白表达水平,结果与各组的0组相比较。结果 A组和B组ADAMTS13mRNA表达水平呈剂量依赖性下降(P<0.05或P<0.01)。与D0组比较,ADAMTS13 mRNA表达水平在D1组下降(P<0.05),在D3组上升(P<0.01)。E、F组ADAMTS13mRNA表达呈辛伐他汀浓度依赖性增加(P<0.05或P<0.01)。结论不同的炎症因子对HK-2上ADAMTS13的表达、合成、分泌起到不同的作用,提示与肾脏局部微循环障碍有关。而辛伐他汀能在存在炎症因子的条件下提高肾小管上皮细胞ADAMTS13的表达。

Objective To investigate the effects of inflammatory cytokines on ADAMTS13 expression in human proximal tubular epithelial cell line（HK-2）and the intervention of simvastatin on it.Methods According to different concentrations,TNF-α0,0.1,1,and 10ng/ml were as groups of A0-A3,IL-6 0,1,10 and 100ng/ml were as groups of B0-B3,IL-4 0,1,5and 10ng/ml were as groups of C0-C3,simvastatin 0,0.1,1and 10μmol/L were as groups of D0-D3.Simvastatin in different concentrations was cocultured with TNF-α10ng/ml（groups of E0-E3）,with IL-6 100ng/ml（groups of F0-F3）,and with IL-4 10ng/ml（groups of G0-G3）.After cultured with HK-2for 24 hours,ADAMTS13mRNA expressions in HK-2were detected by RT-PCR and ADAMTS13 protein levels were detected by Western blot.Results Compared to group 0,ADAMTS13 mRNA in the other TNF-αgroups and IL-6groups were decreased in a dose-dependent manner（P〈0.05 or P〈0.01）.Compared with group D0,ADAMTS13 mRNA decreased in group D1,but increased in group D3（P〈0.01）.ADAMTS13 mRNA expression in groups of E and F increased in a dose-dependent manner（P〈0.05 or P〈0.01）.Conclusion Different inflammatory factors appeare to have different effects on the expression and secretion of ADAMTS13 in HK-2.Simvastatin increases ADAMTS13 mRNA expression in HK-2.