胃癌BLU基因启动子区高甲基化的临床意义

Clinical significance of promoter hypermethylation of BLU gene in gastric cancer

目的研究BLU基因启动子区高甲基化在胃癌发生发展中的作用。方法应用甲基化特异性PCR技术分别检测92例胃癌组织与相应的癌旁组织以及79例胃癌患者与79例正常人外周血BLU基因甲基化水平,分析BLU基因甲基化与临床病理参数的相关性。结果胃癌组织BLU基因甲基化率高于癌旁组织(67.39%vs.52.17%)(P<0.05)。BLU基因甲基化与患者性别、年龄、肿瘤大小、分化程度、TNM分期和浸润深度之间无明显相关性(P>0.05),但与肿瘤淋巴结转移密切相关(P<0.05)。胃癌患者外周血BLU基因甲基化率与正常人外周血比较差异无统计学意义(63.29%vs.56.96%)(P>0.05)。结论 BLU基因启动子的CpG岛在胃癌组织存在高甲基化现象,提示BLU基因的高甲基化可能与抑癌基因被失活从而导致胃癌的发生有关。BLU甲基化可能参与胃癌淋巴结转移。

Objective To investigate the role of promoter hypermethylation of BLU gene in the development and progression of gastric cancer.Methods The methylation level of BLU gene was detected by methylation-specific PCR in 92 samples of gastric cancer tissues（group A）,92 samples of tumor-adjacent normal tissues（group B）,79 samples of peripheral blood from gastric cancer patients（group C）and 79 samples of peripheral blood from healthy people（group D）.The relationship between BLU methylation and clinicopathologic characteristics was analyzed.Results The positive rate of BLU methylation was higher in group A than that in group B（67.39% vs.52.17%）（P〈0.05）.BLU methylation was not significantly correlated with sex,age,tumor size,differentiation degree,TNM stage and depth of infiltration（P〉0.05）,but was closely related with lymph node metastasis（P〈0.05）.There was no significant difference in the positive rate of BLU methylation between group C and group D（63.29% vs.56.96%）（P 〉0.05）.Conclusion The promoter hypermethylation of BLU gene exists in gastric cancer,which indicates that the promotor hypermethylation of BLU gene is associated with an inactivation of BLU gene and tumorigenesis.BLU methylation may participate in the lymphatic metastasis of gastric cancer.