高温高压对壳聚糖/甘油磷酸钠温敏凝胶释药性能的影响

The effect of high temperature and pressure on drug release of chitosan/glycerophosphate thermosensitive hydrogel

目的:研究高温高压后的壳聚糖/甘油磷酸钠凝胶温敏、释药性能。方法:观察不同比例的壳聚糖和甘油磷酸钠混合液的凝胶时间、p H值变化。粘度测试比较不同比例壳聚糖/甘油磷酸钠混合液粘度变化。在37℃形成凝胶后,观察该体系作为奥硝唑药物模型载体的释药性能。结果:随着壳聚糖/甘油磷酸钠混合液中甘油磷酸钠的比例增加,溶液p H值增加,在37℃下,壳聚糖/甘油磷酸钠凝胶时间缩短,但当壳聚糖/甘油磷酸比例为2:1、1:1时,溶液不凝胶。粘度实验表明,4种比例(9:1、8:1、7:1、6:1)壳聚糖/甘油磷酸溶液随着时间增加,粘度明显增加,到10 min后粘度基本不变化,9:1比例组粘度最大为(53±0.9)Pa·s。药物释放实验表明,以奥硝唑为释药模型,该凝胶可持续释放200 min以上。结论:改进消毒方法后的壳聚糖/甘油磷酸钠,增加甘油磷酸钠比例可以提高溶液p H值,可在37℃下快速形成凝胶。该凝胶具有温敏性,具有缓慢释放药物能力。

Objective：To investigate thermosensitivity and drug release of chitosan/glycerphosphate （CS/GPS） by improved method of sterilization. Method：The gel time,viscosity and pH of different proportion of CS/GPS solution were investigated. The drug release of the gel formed at 37℃was evaluated by Ornidazole as a model drug. Result：The more the ratio of glycerophosphate in CS/GPS was,the higher the pH of CS/GPS was,and the shorter the gel time was at 37℃. But when the ratio of CS/GPS was 2：1 and 1：1,the solution didn＆#39;t form gel. The viscosity experiment showed that As the time increased,the viscosity of four proportions （9：1,8：1,7：1,6：1） CS/GPS solution increased distinctly. The viscosity didn‘t change at 10 min,the proportion of 9：1 was highest at （53±0.9）Pa·s. Drug release experiment showed that using ornidazole as release model,the gel could release over 200min. Conclusion：when CS/GPS was sterilized by improved method,in-creased the ratio of GPS could raise the pH of CS/GPS and rapidly formed gel at 37℃. The gel had thermositivity and sus-tained capacity of release drug.