TSCC中Id-1,HIF-1α的表达意义及与血管生成的关系

Expressions of inhibitor of differentiation-1 and hypoxia-inducible factor-1α tongue squamous cell carcinoma and its correlation with angiogenesis

目的:探讨舌鳞状细胞癌(Tongue squamous cell carcinoma,TSCC)组织中分化抑制因子-1(Inhibitor of differentiation-1,Id-1)和乏氧诱导因子-1α(Hypoxia-inducible factor-1α,HIF-1α)的表达及与微血管密度(microvessel density,MVD)的关系。方法:应用免疫组织化学SP法检测65例TSCC中Id-1,HIF-1α,CD34的表达并计数MVD。结果:65例TSCC中Id-1有34例(52.3﹪)阳性表达,43例HIF-1α(66.2﹪)阳性表达。Id-1和HIF-1α表达与TSCC病理分级,淋巴结转移及TNM分期显著相关(P<0.05,χ2检验),并且与MVD值相关(P<0.05)。在TSCC中Id-1和HIF-1α表达呈正相关(P<0.05)。结论:Id-1和HIF-1α与TSCC的恶性进展密切相关,共同参与肿瘤血管生成的过程。联合检测可作为判断TSCC恶性潜能的重要生物指标。

Objective：To investigate the correlation between the expression of inhibitor of differentiation-1（Id-1） and Hypoxia-inducible factor-1 （HIF-1α） and microvessel density （MVD） in Tongue squamous cell carcinoma （TSCC）. Method：Id-1 and HIF-1α expression were investigated by means of immunohistochemistry in samples from 65 cases of TSCC.The density of the microvessels immunohistochemically stained by CD34 antibody,respectively,was calculated. Result：Id-1 positive expression occurred in 34 out of the 65 tumor samples （52.3 %）,while HIF-1α positive expression was ob-served in 43 out of the 65 tumor samples（66.3%）.The positive expression of Id-1 and HIF-1αwere significantly associated with pathological grad,lymph node metastasis and TNM stage （P〈0.05,Chi-square test）.TSCC with positive Id-1 and HIF-1αexpression have higher MVD（P〈0.05,Mann-Whitney U-test）. There were positive corelations between the expression of Id-1 and HIF-1α（P〈0.05,Chi-square test）. Conclusion：Id-1 and HIF-1αtake part in the malignant progression and an-giogenesis of TSCC.They can be considered as biological indicators of malignant potential in TSCC.