摘要
目的 探讨纳米氧化锌(zinc oxide nanoparticles,ZnO-NPs)对人冠状动脉内皮细胞血红素氧合酶-1(heme oxygenase-1,HO-1)和血小板内皮细胞黏附分子-1(platelet endothelial cell adhesion molecules-1,PECAM-1)蛋白表达的影响.方法 采用噻唑蓝实验(MTT比色法)检测ZnO-NPs(12.5、25、50、70、80、90、100、200 μg/ml处理后)对人冠状动脉内皮细胞存活率的影响.用不同浓度ZnO-NPs(0、10、20、40 μg/ml)刺激人冠状动脉内皮细胞24 h后,用ELISA法检测细胞培养上清中HO-1及PECAM-1蛋白的表达.结果 12.5、25、50、70、80、90、100、200 μg/ml ZnO-NPs处理后,人冠状动脉内皮细胞的存活率分别为89.76%、83.61%、63.10%、53.20%、48.11%、42.35%、38.06%、25.44%.0、10、20、40μg/ml ZnO-NPs刺激后,人冠状动脉内皮细胞HO-1蛋白的表达量分别为(0.041±0.011)、(0.512±0.076)、(0.906±0.059)、(1.062±0.089) ng/L,两两比较,差异均有统计学意义(P<0.05),ZnO-NPs浓度与HO-1蛋白表达存在剂量-效应关系.0、10、20、40 μg/ml ZnO-NPs刺激后,人冠状动脉内皮细胞PECAM-1蛋白的表达量分别为(7.966±0.046)、(7.993±0.036)、(8.629±0.052)、(8.811±0.039) μg/L,当ZnO-NPs浓度为20、40 μg/ml时,与空白对照组比较,PECAM-1蛋白的表达均上升,差异均有统计学意义(P<0.05).结论 ZnO-NPs能够降低人冠状动脉内皮细胞存活率,并可诱导人冠状动脉内皮细胞HO-1和PECAM-1蛋白表达上调.
Objective To explore the protein expression of heme oxygenase-1 (HO-1) and platelet endothelial cell adhesion molecules-1 (PECAM-1) in human coronary artery endothelial cells induced with Zinc Oxide Nanoparticle (ZnO-NPs).Methods MTT assay was used to determine the cell viability of ZnO-NPs.Levels of HO-1 and PECAM-1 protein in culture supernatants were measured using ELISA after human coronary artery endothelial cells were treated with different concentrations (0,10,20,40μg/ml) of ZnO-NPs for 24 h.Results The cell viability of human coronary artery endothelial cells in each group was 89.76%,83.61%,63.10%,53.20%,48.11%,42.35%,38.06%,25.44% respectively when treated with different concentrations of ZnO-NPs (12.5,25,50,70,80,90,100,200μg/ml).Protein levels of HO-1 (ng/L) in each group were 0.041±0.011,0.512±0.076,0.906±0.059,1.062±0.089 respectively after the stimulation of different concentrations of ZnO-NPs (0,10,20,40μg/ml).Comparisons in each group were statistically significant (P〈0.05).Protein levels of PECAM-1 (μg/L) in each group were 7.966±0.046,7.993±0.036,8.629±0.052,8.811 ±0.039respectively after the stimulation of different concentrations of ZnO-NPs (0,10,20,40 μg/ml).Compared with the control group,protein levels of PECAM-1 increased (P〈0.05) when the concentration of ZnO-NPs was 20 μg/ml or 40 μg/ml.Conclusion ZnO-NPs stimulation could inhibit the viability of human coronary artery endothelial cells and upregulate the protein expression of HO-1 and PECAM-1.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
2015年第1期11-14,共4页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
国家自然科学基金项目资助(81373030)