纳米氧化锌对人冠状动脉内皮细胞血红素氧合酶-1和血小板内皮细胞黏附分子-1表达的影响

The protein expression of heme oxygenase-1 and platelet endothelial cell adhesion molecules-1 in human coronary artery endothelial cell induced by zinc oxide nanoparticle

目的 探讨纳米氧化锌（zinc oxide nanoparticles,ZnO-NPs）对人冠状动脉内皮细胞血红素氧合酶-1（heme oxygenase-1,HO-1）和血小板内皮细胞黏附分子-1（platelet endothelial cell adhesion molecules-1,PECAM-1）蛋白表达的影响.方法 采用噻唑蓝实验（MTT比色法）检测ZnO-NPs（12.5、25、50、70、80、90、100、200 μg/ml处理后）对人冠状动脉内皮细胞存活率的影响.用不同浓度ZnO-NPs（0、10、20、40 μg/ml）刺激人冠状动脉内皮细胞24 h后,用ELISA法检测细胞培养上清中HO-1及PECAM-1蛋白的表达.结果 12.5、25、50、70、80、90、100、200 μg/ml ZnO-NPs处理后,人冠状动脉内皮细胞的存活率分别为89.76％、83.61％、63.10％、53.20％、48.11％、42.35％、38.06％、25.44％.0、10、20、40μg/ml ZnO-NPs刺激后,人冠状动脉内皮细胞HO-1蛋白的表达量分别为（0.041±0.011）、（0.512±0.076）、（0.906±0.059）、（1.062±0.089） ng/L,两两比较,差异均有统计学意义（P＜0.05）,ZnO-NPs浓度与HO-1蛋白表达存在剂量-效应关系.0、10、20、40 μg/ml ZnO-NPs刺激后,人冠状动脉内皮细胞PECAM-1蛋白的表达量分别为（7.966±0.046）、（7.993±0.036）、（8.629±0.052）、（8.811±0.039） μg/L,当ZnO-NPs浓度为20、40 μg/ml时,与空白对照组比较,PECAM-1蛋白的表达均上升,差异均有统计学意义（P＜0.05）.结论 ZnO-NPs能够降低人冠状动脉内皮细胞存活率,并可诱导人冠状动脉内皮细胞HO-1和PECAM-1蛋白表达上调.

Objective To explore the protein expression of heme oxygenase-1 （HO-1） and platelet endothelial cell adhesion molecules-1 （PECAM-1） in human coronary artery endothelial cells induced with Zinc Oxide Nanoparticle （ZnO-NPs）.Methods MTT assay was used to determine the cell viability of ZnO-NPs.Levels of HO-1 and PECAM-1 protein in culture supernatants were measured using ELISA after human coronary artery endothelial cells were treated with different concentrations （0,10,20,40μg/ml） of ZnO-NPs for 24 h.Results The cell viability of human coronary artery endothelial cells in each group was 89.76%,83.61%,63.10%,53.20%,48.11%,42.35%,38.06%,25.44% respectively when treated with different concentrations of ZnO-NPs （12.5,25,50,70,80,90,100,200μg/ml）.Protein levels of HO-1 （ng/L） in each group were 0.041±0.011,0.512±0.076,0.906±0.059,1.062±0.089 respectively after the stimulation of different concentrations of ZnO-NPs （0,10,20,40μg/ml）.Comparisons in each group were statistically significant （P〈0.05）.Protein levels of PECAM-1 （μg/L） in each group were 7.966±0.046,7.993±0.036,8.629±0.052,8.811 ±0.039respectively after the stimulation of different concentrations of ZnO-NPs （0,10,20,40 μg/ml）.Compared with the control group,protein levels of PECAM-1 increased （P〈0.05） when the concentration of ZnO-NPs was 20 μg/ml or 40 μg/ml.Conclusion ZnO-NPs stimulation could inhibit the viability of human coronary artery endothelial cells and upregulate the protein expression of HO-1 and PECAM-1.