Pgp、Top-Ⅱ、EGFR在结直肠癌中的表达及意义

Expression and significance of P-glycoprotein,Topoisomerase Ⅱ and epidermal growth factor receptor in colorectal cancer

目的:分析结直肠癌多药耐药蛋白P糖蛋白、拓扑异构酶-Ⅱ与表皮生长因子受体(EGFR)蛋白表达的关系,探讨原发性多药耐药患者肿瘤组织中是否存在西妥昔单抗的靶点。方法:用免疫组织化学Eli VisionTM法检测结直肠癌组织Pgp、Top-Ⅱ、EGFR蛋白表达,分析其与临床病理参数间的关系。结果:结直肠癌标本中,Pgp、Top-Ⅱ和EGFR的阳性率分别为85.9%、84.8%和76.1%。Top-Ⅱ在无远隔转移组的表达率显著高于远隔转移组(P<0.05);Pgp、EGFR表达与患者性别、年龄及肿瘤发生部位、浸润深度、TNM分期均无显著相关关系。耐药蛋白Pgp、Top-Ⅱ与西妥昔单抗靶点蛋白EGFR表达无显著相关关系(P>0.05)。结论:Top-Ⅱ在无远隔转移的结直肠癌组织表达较高;包括传统化疗耐药与敏感患者在内的大部分结直肠癌患者肿瘤组织内均有西妥昔单抗的靶点。

Objective ：To discuss the possibility of drug target of Cetuximab exist in patients with chemotherapy re- sistance by analyzing the association between drug resistance proteins P -glycoprotein（Pgp） ,Topoisomerase Ⅱ （Top -Ⅱ ） and epidermal growth factor receptor（EGFR）. Methods：The expressions of Pgp, Top - Ⅱ , EGFR were detec- ted by immunohistochemistry. The relationship between the expressions of drug resistance proteins and EGFR was ana- lyzed by Spearman correlation analysis and Chi - square test. Results： The expression rates of Pgp, Top - Ⅱ and EG- FR in colorectal cancer were 85.9% ,84.8% and 76.1% ,respectively. The Top -Ⅱ expression rate was statistically higher in non - metastasis group than that in metastasis group（ P 〈 0.05 ）. The expressions of Pgp, EGFR were not re- lated to patient gender, age, tumor invasion or lymph node metastasis. No association was found between drug resist- ance proteins and EGFR（P 〉 0.05 ）. Conclusion ： The expression rate of Top - Ⅱ was statistically higher in non - metastasis group than that in metastasis group. Most patients,including drug resistance patients and the ones are sensi- five to chemotherapy,have the drug target of Cetuximab.