Ech1的表达下调对小鼠肝癌淋巴道转移的影响及其可能机制

The effect of Ech1 downregulation on lymphatic metastasis of mouse heptocarcinoma and the mechanism

目的:探讨Ech1表达下调对小鼠肝癌淋巴道转移率的影响,及其与肿瘤相关蛋白Anxa7的相互关系。方法:将Ech1基因表达下调的Hca-F细胞植入小鼠足垫,28天后观察小鼠成瘤及转移情况。在Ech1基因表达下调的Hca-F细胞和对照Hca-F细胞中,通过Western blot检测Anxa7表达变化,同时在Anxa7表达下调Hca-F细胞和对照Hca-F细胞中通过Western blot检测Ech1表达变化。结果:下调Ech1基因的表达,使肿瘤细胞体内淋巴结转移率明显降低。Ech1表达下调后,肿瘤转移相关蛋白Anxa7表达增加;Anxa7表达下调后,Ech1蛋白表达下降。结论:肿瘤细胞Ech1基因表达水平与小鼠肝癌淋巴道转移密切相关,Ech1和Anxa7在Hca-F肿瘤淋巴道转移中可能相互作用。

Objective：To observe the lymphatic metastasis of mouse heptocarcinoma when Echl protein was down - regulated in Hca - F cells, and the expression of Ech 1 protein and the tumor related protein Anxa7 in FAnxa7-down cell and FEchl-down, cell. Methods： FEchl downregulatedcell （1 × 106） were inoculated into the feet pad of 615 mice subcutaneously. On the 28th day of postinoculation, the mice were euthanized and their lymph nodes were isola- ted, weighed, sectioned, and stained with hematoxylin and eosin. The expression of Echl and Anxa7 were observed by Western blot analysis in FAnxa7-down cell and FEchl -dow.cell. Results： Down - regulation of Echl could inhibit the lym- phatic metastasis of Hca - F cells, and increase expression of the tumor related protein Anxa7, while down - regulated Anxa7 could decrease the expression of Echl. Conclusion：Echl is an important factor in the lymphatic metastasis of tumors, Echl and Anxa7 may work together during the lymphatic metastasis of Hca- F cell.