扁桃体鳞癌中HPV感染与EGFR、VEGF表达的相关性研究

The correlation of HPV infection with the expression of epidermal growth factor receptor( EGFR) and vascular endothelial growth factor( VEGF) in squamous cell carcinomas of the tonsil

目的:研究扁桃体鳞癌(squamous cell carcinomas of the tonsil tonsillar,SCCs)中人乳头瘤病毒(human papillomavirus,HPV)感染与血管内皮生长因子(vascular endothelial growth factor,VEGF)和表皮生长因子受体(epidermal growth factor,EGFR)表达的关系,探讨HPV与EGFR和VEGF在扁桃体鳞癌发生中的交互效应。方法:采用多重实时荧光定量聚合酶链反应(multiplex real-time polymerase chain reaction,MT-PCR)检测85例扁桃体鳞癌HPV DNA及型别,通过HPV DNA分析进行HPV感染状况的测定;同时采用半定量免疫组化检测HPV(+)组和HPV(-)组中VEGF、EGFR蛋白表达情况。结果:扁桃体鳞癌中HPV感染率为49.4%(42/85),HPV-16型占所有感染者的比例为90.5%(38/42),明显高于其他型别;HPV(+)组EGFR蛋白表达明显低于HPV(-)组(P值均<0.01);HPV(+)组与HPV(-)组VEGF蛋白表达无明显差异;此外,VEGF表达与EGFR、患者的年龄、性别、TNM分期、组织学分级均无明显相关性。结论:HPV感染与扁桃体鳞癌的发生存在相关性,HPV相关的扁桃体鳞癌中,HPV基因可能通过改变EGFR表达致癌,为HPV致癌机理进一步研究提供一个新的视角,具有重要的理论意义。

Objective：To study the relationship between human papillomavirus （HPV） infection and the expres- sion of vascular endothelial growth factor（VEGF） , epidermal growth factor（EGFR） in 85 squamous cell carcinomas of the tonsil（ tonsillar SCCs）. To investigate their significance in the oncogenesis of tonsillar cancer. Methods： HPV status was determined by HPV multiplex real - time polymerase chain reaction（ MT - PCR）. Expression of VEGF and EGFR in HPV （ ＋ ） and HPV （ - ） group was assessed by semiquantitative immunohistochemistry. Results：The infec- tion rate of HPV in tonsillar SCCs was 49.4% （42/85）. The infection rate of HPV - 16 was 90.5% （38/42） in all positive specimens was significantly higher than other HPV types. The expression of EGFR in HPV （ - ） group was significantly higher than that in HPV（ ＋ ） group（ P 〈 0.01 ）. The expression of VEGF had no significant difference between HPV（ ＋ ） group and HPV（ - ） group. There were no associations between VEGF and EGFR expression, gender, age,TNM stage or histology grade. Conclusion： HPV infection has significant correlation with the oncogenesis of tonsillar SCCs. In HPV - positive tonsillar SCCs, HPV gene may change EGFR expression.